Inducing effect of skeletal muscle extracts on the appearance of calbindin-immunoreactive dorsal root ganglion cells in culture.

Détails

ID Serval
serval:BIB_8DD6D92FA3FF
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Inducing effect of skeletal muscle extracts on the appearance of calbindin-immunoreactive dorsal root ganglion cells in culture.
Périodique
Neuroscience
Auteur(s)
Barakat I., Droz B.
ISSN
0306-4522
Statut éditorial
Publié
Date de publication
1989
Peer-reviewed
Oui
Volume
28
Numéro
1
Pages
39-47
Langue
anglais
Résumé
Calbindin D-28k is a calcium-binding protein which is not expressed by dorsal root ganglion cells cultured from 6-day-old (E6) chick embryos. When soluble muscle extracts from embryos at E11, E18 or chickens 2 weeks after hatching were added immediately after seeding, dorsal root ganglia cells grown at E6 displayed neuronal subpopulations expressing calbindin immunoreactivity with time; the effect of muscle extract on the percentage of calbindin-immunoreactive dorsal root ganglia cells followed a dose-response curve. When muscle extract was added to cultures after a 3 day delay, the percentage of calbindin-expressing neurons was unchanged. The effect produced by muscle extract and, to a lesser degree, skin extract on the appearance of calbindin-positive neurons was not reproduced by brain or liver extracts while all four exerted a trophic action on cultured neurons. Hence it is assumed that muscle extract contains a factor which produces an inductive effect on the initiation of calbindin-expression by uncommitted subpopulations of sensory neurons rather than a trophic influence on the selective survival of covertly committed neuronal subpopulations. The fact that muscle extract promoted calbindin expression by dorsal root ganglia cells in neuron-enriched as well as in mixed dorsal root ganglion cell cultures indicates that the factor would act directly on sensory neurons rather than indirectly through mediation of non-neuronal cells. Since the active muscular factor was non-dialysable, heat-inactivated, trypsin-sensitive and retained by molecular filters with a cut-off of 30 K, this factor is probably a protein.
Mots-clé
Animals, Calcium-Binding Protein, Vitamin D-Dependent, Cell Survival, Cells, Cultured, Chick Embryo, Chickens, Ganglia, Spinal, Immunohistochemistry, Muscles, Neurons, Tissue Extracts
Pubmed
Web of science
Création de la notice
30/03/2009 10:26
Dernière modification de la notice
03/03/2018 19:17
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