The heart communicates with the endothelium through the guanylyl cyclase-A receptor: acute handling of intravascular volume in response to volume expansion.

Détails

ID Serval
serval:BIB_8DAAA5D6E9CA
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
The heart communicates with the endothelium through the guanylyl cyclase-A receptor: acute handling of intravascular volume in response to volume expansion.
Périodique
Endocrinology
Auteur(s)
Schreier B., Börner S., Völker K., Gambaryan S., Schäfer S.C., Kuhlencordt P., Gassner B., Kuhn M.
ISSN
0013-7227
Statut éditorial
Publié
Date de publication
2008
Volume
149
Numéro
8
Pages
4193-4199
Langue
anglais
Résumé
Atrial natriuretic peptide (ANP) regulates arterial blood pressure and volume. Its guanylyl cyclase-A (GC-A) receptor is expressed in vascular endothelium and mediates increases in cGMP, but the functional relevance is controversial. Notably, mice with endothelial-restricted GC-A deletion [EC GC-A knockout (KO) mice] exhibit significant chronic hypervolemic hypertension. The present study aimed to characterize the endothelial effects of ANP and their relevance for the acute regulation of intravascular fluid volume. We studied the effect of ANP on microvascular permeability to fluorescein isothiocyanate-labeled albumin (BSA) using intravital microscopy on mouse dorsal skinfold chambers. Local superfusion of ANP (100 nm) increased microvascular fluorescein isothiocyanate-BSA extravasation in control but not EC GC-A KO mice. Intravenous infusion of synthetic ANP (500 ng/kg x min) caused immediate increases in hematocrit in control mice, indicating intravascular volume contraction. In EC GC-A KO mice, the hematocrit responses were not only abolished but even reversed. Furthermore, acute vascular volume expansion, which caused release of endogenous cardiac ANP, did not affect resting central venous pressure of control mice but rapidly and significantly increased central venous pressure of EC GC-A KO mice. In cultured lung endothelial cells, ANP provoked cGMP-dependent protein kinase I-mediated phosphorylation of vasodilator-stimulated phosphoprotein. We conclude that ANP, via GC-A, enhances microvascular endothelial macromolecule permeability in vivo. This effect might be mediated by cGMP-dependent protein kinase I-dependent phosphorylation of vasodilator-stimulated phosphoprotein. Modulation of transcapillary protein and fluid transport may represent one of the most important hypovolemic actions of ANP.
Mots-clé
Animals, Atrial Natriuretic Factor, Blood Volume, Capillary Permeability, Cell Adhesion Molecules, Cell Communication, Cells, Cultured, Cyclic GMP-Dependent Protein Kinases, Endothelium, Vascular, Heart, Hematocrit, Mice, Mice, Knockout, Microfilament Proteins, Myocardium, Phosphoproteins, Phosphorylation, Receptors, Atrial Natriuretic Factor, Venous Pressure
Pubmed
Web of science
Open Access
Oui
Création de la notice
25/06/2008 11:56
Dernière modification de la notice
08/05/2019 21:49
Données d'usage