Transient promoter formation: a new feedback mechanism for regulation of IS911 transposition

Détails

ID Serval
serval:BIB_8BF301D1833D
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Transient promoter formation: a new feedback mechanism for regulation of IS911 transposition
Périodique
EMBO Journal
Auteur(s)
Duval-Valentin  G., Normand  C., Khemici  V., Marty  B., Chandler  M.
ISSN
0261-4189 (Print)
Statut éditorial
Publié
Date de publication
10/2001
Volume
20
Numéro
20
Pages
5802-11
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Oct 15
Résumé
IS911 transposition involves a free circular transposon intermediate where the terminal inverted repeat sequences are connected. Transposase synthesis is usually driven by a weak promoter, p(IRL), in the left end (IRL). Circle junction formation creates a strong promoter, p(junc), with a -35 sequence located in the right end and the -10 sequence in the left. p(junc) assembly would permit an increase in synthesis of transposase from the transposon circle, which would be expected to stimulate integration. Insertion results in p(junc) disassembly and a return to the low p(IRL)- driven transposase levels. We demonstrate that p(junc) plays an important role in regulating IS911 transposition. Inactivation of p(junc) strongly decreased IS911 transposition when transposase was produced in its natural configuration. This novel feedback mechanism permits transient and controlled activation of integration only in the presence of the correct (circular) intermediate. We have also investigated other members of the IS3 and other IS families. Several, but not all, IS3 family members possess p(junc) equivalents, underlining that the regulatory mechanisms adopted to fine-tune transposition may be different.
Mots-clé
Bacterial Proteins/genetics/physiology DNA Transposable Elements/*genetics DNA, Bacterial/genetics DNA, Circular/genetics Escherichia coli/genetics *Escherichia coli Proteins Feedback Genetic Vectors/genetics Kinetics Models, Genetic Mutagenesis, Site-Directed Promoter Regions (Genetics)/*physiology Recombinant Fusion Proteins/physiology Regulatory Sequences, Nucleic Acid Transposases/*physiology
Pubmed
Web of science
Open Access
Oui
Création de la notice
28/01/2008 10:31
Dernière modification de la notice
08/05/2019 21:44
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