High-angle annular dark-field stem imaging of immunogold labels

Détails

ID Serval
serval:BIB_8BEC92336549
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
High-angle annular dark-field stem imaging of immunogold labels
Périodique
Scanning Microscopy
Auteur(s)
Otten M.T., Stenzel D.L., Cousens D.R., Humbel B.M., Leunissen J.L.M., Stierhof Y.D., Busing W.M.
ISSN
0161-0457
Statut éditorial
Publié
Date de publication
1992
Volume
14
Numéro
5
Pages
282-289
Langue
anglais
Résumé
High-angle annular dark-field (HAADF) STEM imaging is a sensitive and efficient technique for detecting immunogold labels. Larger (5-15 nm) gold labels can be distinguished clearly from the heavy metal stain on tissue sections, but for smaller (<5 nm) labels the distinction is less clear. It is not possible to differentiate between ultra-small (<1-3 nm) labels and stain, but the HAADF image shows sufficient contrast so specimens can remain unstained. On a TEM/STEM equipped with a LaB6 filament the smallest labels that are detectable are 1-2 nm. Efficient detection of ultra-small labels requires a field emission microscope.
Web of science
Création de la notice
18/10/2012 14:35
Dernière modification de la notice
03/03/2018 19:12
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