Cellular immunity to merozoite surface protein 2 (FC27 and 3D7) in Papua New Guinean children. Temporal variation and relation to clinical and parasitological status

Détails

ID Serval
serval:BIB_8A4C41DDC4E3
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Cellular immunity to merozoite surface protein 2 (FC27 and 3D7) in Papua New Guinean children. Temporal variation and relation to clinical and parasitological status
Périodique
Parasite Immunology
Auteur(s)
al-Yaman  F., Genton  B., Taraika  J., Anders  R., Alpers  M. P.
ISSN
0141-9838 (Print)
Statut éditorial
Publié
Date de publication
05/1997
Volume
19
Numéro
5
Pages
207-14
Notes
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S. --- Old month value: May
Résumé
A prospective study in 207 children aged 0.5-15 years was carried out in a highly endemic area of Papua New Guinea to examine the relationship between cellular responses to Plasmodium falciparum merozoite surface protein 2 (MSP2) and malaria infection and morbidity. In vitro proliferation, IFN-gamma and IL-4 induction were measured against two recombinant proteins of MSP2, FC27 and 3D7 as well as against a form of the 3D7 MSP2 lacking the central repetitive sequences (d3D7). The prevalence of proliferative response was generally low, 6% for FC27, 9% for 3D7 and 11% for d3D7. A higher prevalence of IL-4 response was obtained being 27% for FC27, 34% for 3D7 and 30% for d3D7 while the prevalence of IFN-gamma response was 13%, 15% and 18%, respectively. There was no correlation between age and proliferative responses; in contrast cytokine production increased with age for all three antigens. When proliferation or stimulation of either cytokine was used to assess T-cell activation the frequency of responders increased to 39%, 47% and 46% for FC27, 3D7 and d3D7 respectively. Analysis of the relation of T cell responses to concurrent infection and morbidity showed that lymphoproliferative response only to d3D7 was significantly associated with parasitaemia; while lymphoproliferative responses to all 3 MSP2 antigens were highest in the group of clinical malaria cases. There was no significant correlation between proliferation or cytokine production to MSP2 and concurrent or subsequent malaria morbidity.
Mots-clé
Adolescent Animals *Antigens, Protozoan Antigens, Surface/*immunology Child Child, Preschool Cross-Sectional Studies Cytokines/*biosynthesis Endemic Diseases Humans Infant Interferon Type II/biosynthesis Interleukin-4/biosynthesis Lymphocyte Activation Malaria, Falciparum/epidemiology/*immunology Papua New Guinea/epidemiology Plasmodium falciparum/*immunology Prospective Studies Protozoan Proteins/*immunology Recombinant Fusion Proteins/immunology T-Lymphocytes/*immunology
Pubmed
Web of science
Création de la notice
28/01/2008 12:48
Dernière modification de la notice
03/03/2018 19:09
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