Progesterone is an autocrine/paracrine regulator of human granulosa cell survival in vitro.

Détails

ID Serval
serval:BIB_8A3AD9A9D794
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Progesterone is an autocrine/paracrine regulator of human granulosa cell survival in vitro.
Périodique
Annals of the New York Academy of Sciences
Auteur(s)
Makrigiannakis A., Coukos G., Christofidou-Solomidou M., Montas S., Coutifaris C.
ISSN
0077-8923 (Print)
ISSN-L
0077-8923
Statut éditorial
Publié
Date de publication
2000
Volume
900
Pages
16-25
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, P.H.S.Publication Status: ppublish
Résumé
Ovarian follicles are composed of granulosa cells (GC), which undergo apoptosis within 24 hours of culture in serum-free medium. The present study was designed to assess the role of progesterone in regulating human GC survival. Human GC were isolated from follicular aspirates of women undergoing in vitro fertilization. GC were then cultured for 24 hours in serum-free media supplemented with progesterone and/or the progesterone antagonist RU486 and dexamethasone. Cells were then fixed and assessed for apoptosis by in situ end labeling of DNA fragments, cell cycle analysis of DNA content, and electron microscopy. When compared with controls, progesterone reduced and RU486 increased the percentage of apoptotic GC (p < 0.05), whereas dexamethasone had no effect. In addition, RU486 inhibited the protective effect of progesterone on GC survival (p < 0.05). Taken together, these data indicate that progesterone inhibits human GC apoptosis, and this effect is mediated through the progesterone receptor.
Mots-clé
Adult, Apoptosis/drug effects, Autocrine Communication, Cell Cycle, Cell Survival, Cells, Cultured, DNA/analysis, Dexamethasone/pharmacology, Dose-Response Relationship, Drug, Female, Flow Cytometry, Granulosa Cells/chemistry, Granulosa Cells/physiology, Hormone Antagonists/pharmacology, Humans, In Situ Nick-End Labeling, Microscopy, Electron, Mifepristone/pharmacology, Paracrine Communication, Progesterone/antagonists & inhibitors, Progesterone/physiology, Receptors, Progesterone/physiology
Pubmed
Web of science
Création de la notice
14/10/2014 12:43
Dernière modification de la notice
03/03/2018 19:09
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