Article: article from journal or magazin.
Progesterone is an autocrine/paracrine regulator of human granulosa cell survival in vitro.
Annals of the New York Academy of Sciences
Publication types: Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, P.H.S.Publication Status: ppublish
Ovarian follicles are composed of granulosa cells (GC), which undergo apoptosis within 24 hours of culture in serum-free medium. The present study was designed to assess the role of progesterone in regulating human GC survival. Human GC were isolated from follicular aspirates of women undergoing in vitro fertilization. GC were then cultured for 24 hours in serum-free media supplemented with progesterone and/or the progesterone antagonist RU486 and dexamethasone. Cells were then fixed and assessed for apoptosis by in situ end labeling of DNA fragments, cell cycle analysis of DNA content, and electron microscopy. When compared with controls, progesterone reduced and RU486 increased the percentage of apoptotic GC (p < 0.05), whereas dexamethasone had no effect. In addition, RU486 inhibited the protective effect of progesterone on GC survival (p < 0.05). Taken together, these data indicate that progesterone inhibits human GC apoptosis, and this effect is mediated through the progesterone receptor.
Adult, Apoptosis/drug effects, Autocrine Communication, Cell Cycle, Cell Survival, Cells, Cultured, DNA/analysis, Dexamethasone/pharmacology, Dose-Response Relationship, Drug, Female, Flow Cytometry, Granulosa Cells/chemistry, Granulosa Cells/physiology, Hormone Antagonists/pharmacology, Humans, In Situ Nick-End Labeling, Microscopy, Electron, Mifepristone/pharmacology, Paracrine Communication, Progesterone/antagonists & inhibitors, Progesterone/physiology, Receptors, Progesterone/physiology
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