cDNA and protein characterization of human MAGE-10

Détails

ID Serval
serval:BIB_8757850ADF29
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
cDNA and protein characterization of human MAGE-10
Périodique
International Journal of Cancer
Auteur(s)
Rimoldi  D., Salvi  S., Reed  D., Coulie  P., Jongeneel  V. C., De Plaen  E., Brasseur  F., Rodriguez  A. M., Boon  T., Cerottini  J. C.
ISSN
0020-7136 (Print)
Statut éditorial
Publié
Date de publication
09/1999
Volume
82
Numéro
6
Pages
901-7
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Sep 9
Résumé
MAGE genes are frequently expressed in several types of human malignancy and code for antigens recognized by cytotoxic T lymphocytes. We have previously described a monoclonal antibody (MAb), named 6C1, that recognizes the MAGE-1 protein and cross-reacts with a 72-kDa protein present in lysates of melanoma cells such as MZ2-MEL. To identify this protein, we have screened an expression library prepared from MZ2-MEL cells. Several clones that encoded a protein recognized by antibody 6C1 contained a sequence identical to that of MAGE-10, another member of the MAGE-A gene family. Full-length MAGE-10 cDNA clones, obtained after screening additional cDNA melanoma libraries, were found to be approximately 2.5 kb in length. In vitro translation and transient transfection experiments indicated that MAGE-10 codes for a protein of approximately 72 kDa. This product was recognized by MAb 6C1 as well as by a polyclonal serum raised against a MAGE-10 peptide, thus demonstrating its identity with MAGE-10. Analysis of MAGE-10 mRNA by RT-PCR confirmed its presence in testis and placenta but not in other normal tissues. Expression of MAGE-10 in melanoma tumors was found to parallel that of MAGE-1. Western blot analysis with the polyclonal anti-MAGE-10 antibody showed the presence of MAGE-10 in lysates of purified trophoblast cells. Immuno-cytochemistry of cultured melanoma cells indicated that MAGE-10 is a nuclear protein.
Mots-clé
Antibodies, Monoclonal Antigens, Neoplasm/*genetics Blotting, Northern Cloning, Molecular DNA, Complementary Female Gene Library Humans Introns Male Melanoma/*genetics Neoplasm Proteins/chemistry/*genetics/metabolism Organ Specificity Polymerase Chain Reaction Protein Biosynthesis Recombinant Proteins/biosynthesis/chemistry/metabolism Transcription, Genetic Transfection
Pubmed
Web of science
Création de la notice
28/01/2008 12:13
Dernière modification de la notice
20/08/2019 15:46
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