Pro-opiocortin related peptides in human pituitary and ectopic ACTH secreting tumours

Details

Serval ID
serval:BIB_8661CD9C1714
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Pro-opiocortin related peptides in human pituitary and ectopic ACTH secreting tumours
Journal
Clinical Endocrinology
Author(s)
Ratter  S. J., Gillies  G., Hope  J., Hale  A. C., Grossman  A., Gaillard  R., Cook  D., Edwards  C. R., Rees  L. H.
ISSN
0300-0664 (Print)
Publication state
Published
Issued date
03/1983
Volume
18
Number
3
Pages
211-8
Notes
Comparative Study
In Vitro
Journal Article --- Old month value: Mar
Abstract
Basal and stimulated secretion of N-terminal pro-opiocortin (Pro-gamma-MSH), ACTH and LPH from seven pituitary and three ectopic ACTH secreting tumours have been studied in vitro using a perfused isolated cell system. The peptides were shown to be released concomitantly and in equimolar amounts. The pituitary tumours responded to stimulation with rat stalk median eminence extracts (SME) and synthetic AVP. However, peptide release from the ectopic tumours, although pulsatile, remained autonomous. Prior to surgery, gel-chromatographic profiles of plasma immunoreactive ACTH showed only one peak, which eluted in the position of 1-39 ACTH, in patients with the pituitary tumours, but there was a second peak of large molecular weight ACTH present in the plasma from those with the ectopic ACTH syndrome. This second form of ACTH could not be detected in any of the tumour cell column effluents. An eighth pituitary tumour was atypical, in its unusually large size, clinically aggressive nature and spectrum of peptide release. Although peptide release in response to stimulation with SME was similar to that observed with the other pituitary tumours, the chromatography of the plasma ACTH resembled the ectopic plasma pattern, showing two peaks of immunoreactivity.
Keywords
ACTH Syndrome, Ectopic/*metabolism Adrenocorticotropic Hormone/secretion Arginine Vasopressin/pharmacology Cushing Syndrome/metabolism Humans Hydrocortisone/pharmacology Median Eminence/physiology Melanocyte-Stimulating Hormones/*secretion Paraneoplastic Endocrine Syndromes/*metabolism *Peptide Fragments Pituitary Neoplasms/*secretion *Pro-Opiomelanocortin Protein Precursors/*secretion Tissue Extracts/pharmacology beta-Lipotropin/secretion
Pubmed
Web of science
Create date
15/02/2008 16:57
Last modification date
20/08/2019 14:45
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