Role of TNFR1 and TNFR2 in TNF-induced platelet consumption in mice.

Détails

ID Serval
serval:BIB_85A6A7FF9F70
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Role of TNFR1 and TNFR2 in TNF-induced platelet consumption in mice.
Périodique
Journal of Immunology
Auteur(s)
Tacchini-Cottier F., Vesin C., Redard M., Buurman W., Piguet P.F.
ISSN
0022-1767 (Print)
ISSN-L
0022-1767
Statut éditorial
Publié
Date de publication
1998
Volume
160
Numéro
12
Pages
6182-6186
Langue
anglais
Résumé
An injection of TNF in mice induced profound thrombocytopenia, due to an increase of platelet consumption, that was evident after 1 h and lasted for 3 days. This process was evident in mice that were genetically deficient in TNFR2 (p75) but not in mice lacking TNFR1 (p55), indicating that the process is mediated by TNFR1-bearing cells. To explore the site of action of TNF, labeled platelets from TNFR1 -/- or +/+ donors were transferred to TNFR1 -/- or +/+ recipients. TNF induced the consumption of platelets from TNFR1 -/- donors when injected into +/+ recipients, while platelets from +/+ donors were not consumed when present in TNFR1 -/- recipients; this finding indicates that TNF acts on the TNFR1 of host cells but does not act on platelets. The expression of TNFRs is consistent with this interpretation, since TNFRs were not detected on platelets by flow cytometry. In megakaryocytes, the expression of TNFR1 was detected by immunohistochemistry. These results indicate that TNF induces platelet consumption by acting not on platelets directly but on the TNFR1 of other cells, presumably increasing the release of factors with agonist activity for platelets.
Mots-clé
Animals, Antigens, CD/physiology, Blood Platelets/drug effects, Humans, Immunohistochemistry, Mice, Mice, Inbred C57BL, Mice, Inbred CBA, Mice, Knockout, Receptors, Tumor Necrosis Factor/physiology, Receptors, Tumor Necrosis Factor, Type I, Receptors, Tumor Necrosis Factor, Type II, Thrombocytopenia/chemically induced, Thrombocytopenia/immunology, Tumor Necrosis Factor-alpha
Pubmed
Web of science
Création de la notice
24/01/2008 16:09
Dernière modification de la notice
03/03/2018 18:57
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