Disruption of the gene encoding the secreted acid protease (ACP) in the yeast Candida tropicalis
Details
Serval ID
serval:BIB_856C6178941E
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Disruption of the gene encoding the secreted acid protease (ACP) in the yeast Candida tropicalis
Journal
FEMS Microbiology Letters
ISSN
0378-1097 (Print)
Publication state
Published
Issued date
08/1992
Volume
74
Number
2-3
Pages
149-56
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Aug 15
Research Support, Non-U.S. Gov't --- Old month value: Aug 15
Abstract
The gene for the secreted acid protease (ACP), a potential virulence factor of Candida species, was inactivated in Candida tropicalis by gene disruption. The disruption was performed by cotransformation of an ade2 C. tropicalis mutant with a linear DNA fragment carrying a deletion in ACP, and the replicative vector pMK16 which carries a selectable ADE2 gene marker. Few of the transformants exhibited lower protease secretion levels and were shown to have one deleted and one unaffected ACP copy, since C. tropicalis is a diploid yeast. These transformants were rendered homozygotic for this deletion by mild UV-treatment. One of the homozygotic acp deletion mutants obtained was completely devoid of extracellular protease activity and grew poorly on bovine serum albumin-containing medium. This mutant could be complemented by an ACP fragment inserted in pMK16, but also by an acid protease gene isolated from C. parapsilosis.
Keywords
Aspartic Endopeptidases/*genetics/secretion
Candida/enzymology/*genetics/growth & development
Chromosome Deletion
Culture Media
Gene Expression/genetics
Mutation
Serum Albumin, Bovine
Pubmed
Create date
25/01/2008 17:46
Last modification date
20/08/2019 15:44