Hair analysis for buprenorphine and its dealkylated metabolite by RIA and confirmation by LC/ECD.

Détails

ID Serval
serval:BIB_834C5B5B2374
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Hair analysis for buprenorphine and its dealkylated metabolite by RIA and confirmation by LC/ECD.
Périodique
Journal of Forensic Sciences
Auteur(s)
Kintz P., Cirimele V., Edel Y., Jamey C., Mangin P.
ISSN
0022-1198[print], 0022-1198[linking]
Statut éditorial
Publié
Date de publication
1994
Volume
39
Numéro
6
Pages
1497-1503
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Résumé
Hair samples were obtained from 14 subjects admitted 2 or 3 months previously to a detoxification center. All reported an history of intravenous heroin abuse. After decontamination by two dichloromethane washes, about 50 mg hair were pulverized in a ball mill and incubated at 56 degrees C overnight in 1 mL 0.1 HCl. After neutralization, buprenorphine analyzed by RIA was in the range of 0.01 to 0.47 ng/mg. To confirm buprenorphine, liquid chromatography was used. After neutralization, drugs were extracted with toluene at pH 8.5 during a 3-step extraction procedure. A portion of the reconstituted residue was injected into a Lichrosorb CN column, with a mobile phase of phosphate buffer (pH 4.0)-acetonitrile-1-heptane sulfonic acid-butylamine (85:17:2:0.01, v/v). Detection was achieved by coulometry, and the potential of the electrodes was 0.15 and 0.50 V, respectively. Linear calibration curves were obtained from 0.02 to 2.0 ng/mg with a correlation coefficient r > 0.99 for both drugs. The detection limit for the major metabolite was about 0.01 ng/mg and 0.02 ng/mg for buprenorphine, using a 50 mg hair sample. Recovery (at 0.2 ng/mg) was 54 and 62% for norbuprenorphine and buprenorphine, respectively. Drugs concentrations in hair were in the range 0.02-0.59 and not detected--0.15 ng/mg for buprenorphine and norbuprenorphine, respectively. Results suggest that a dose-response relationship exists between the concentration of buprenorphine in hair and the administered dose.
Mots-clé
Adult, Buprenorphine/analogs & derivatives, Buprenorphine/analysis, Calibration, Chromatography, High Pressure Liquid, Dose-Response Relationship, Drug, Female, Hair/chemistry, Heroin, Humans, Male, Mass Spectrometry, Radioimmunoassay, Substance Abuse Detection/methods, Substance-Related Disorders/diagnosis
Pubmed
Création de la notice
19/10/2010 16:26
Dernière modification de la notice
03/03/2018 18:52
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