Glucocorticoids modulate neurotransmitter-induced glycogen metabolism in cultured cortical astrocytes
Details
Serval ID
serval:BIB_8254FCF681AC
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Glucocorticoids modulate neurotransmitter-induced glycogen metabolism in cultured cortical astrocytes
Journal
Journal of Neurochemistry
ISSN
0022-3042 (Print)
Publication state
Published
Issued date
02/2004
Volume
88
Number
4
Pages
900-8
Notes
Comparative Study
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Feb
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Feb
Abstract
Glucocorticoids (GC) are considered as key modulators of glycogen homeostasis in peripheral tissues, but their role in the central nervous system has only partially been characterized. Exposure of primary cultures of cortical astrocytes to dexamethasone (DEX), a synthetic glucocorticoid, results in the reduction of noradrenaline (NA)-induced glycogen synthesis in a concentration-dependent manner with a IC50 of 4.88 nm and a maximum inhibition of 51%. Such an effect is mediated via glucocorticoid receptors (GRs), since it is mimicked by the glucocorticoid analogue RU28362 (100 nm) and prevented by the GR antagonist RU38486 (1 micro m). DEX does not act through alteration of signal transduction mechanisms, as cAMP formation induced by noradrenergic stimulation was unchanged. Moreover, glycogen synthesis was inhibited to the same extent when DEX was applied either together or only after a brief NA application. Neither [3H]2-deoxyglucose uptake nor lactate release was altered by DEX in the presence of NA, demonstrating that inhibition of glycogen synthesis is not a consequence of reduced glucose utilization or availability. Interestingly, enhancement of glycogen synthase activity induced by NA was reduced in the presence of DEX (-27%). These results suggest that GC could have a significant influence on neuroenergetics as they could modulate activity-related changes in brain glycogen metabolism.
Keywords
Androstanols/pharmacology
Animals
Animals, Newborn
Astrocytes/*drug effects/metabolism
Blotting, Northern
Carrier Proteins/genetics/metabolism
Cells, Cultured
Cerebral Cortex/*cytology
Cyclic AMP/metabolism
Deoxyglucose/metabolism
Dose-Response Relationship, Drug
Drug Interactions
Gene Expression/drug effects
Glucocorticoids/metabolism/*pharmacology
Glycogen/*metabolism
Hormone Antagonists/pharmacology
*Intracellular Signaling Peptides and Proteins
Lactase/secretion
Mice
Mifepristone/pharmacology
Neurotransmitter Agents/*pharmacology
Norepinephrine/pharmacology
RNA, Messenger/metabolism
Tritium/metabolism
Pubmed
Web of science
Open Access
Yes
Create date
24/01/2008 14:16
Last modification date
20/08/2019 15:42