The use of the polymerase chain reaction for more sensitive detection of Plasmodium falciparum

Détails

ID Serval
serval:BIB_824B25FB4D34
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
The use of the polymerase chain reaction for more sensitive detection of Plasmodium falciparum
Périodique
Papua New Guinea Medical Journal
Auteur(s)
Felger  I., Tavul  L., Narara  A., Genton  B., Alpers  M., Beck  H. P.
ISSN
0031-1480 (Print)
Statut éditorial
Publié
Date de publication
03/1995
Volume
38
Numéro
1
Pages
52-6
Notes
Comparative Study
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S. --- Old month value: Mar
Résumé
The prevalence of Plasmodium falciparum in children and adults living in a malaria-endemic area in Papua New Guinea was determined by microscopy and by polymerase chain reaction (PCR). The sensitivity of detecting P. falciparum infections increased two-fold with PCR. Undetected infections by microscopy were more frequent in adults (including adolescents) than in children. Detecting this subpatent parasitaemia by PCR resulted in an equal P. falciparum prevalence in children and adults; in children the parasitaemia rate increased from 32% to 48% and in adults from 23% to 47%. In more than 50% of all blood samples positive for P. vivax and P. malariae an underlying P. falciparum infection remained undetected by microscopy. The introduction of PCR has opened up new possibilities in malaria diagnosis and research.
Mots-clé
Adolescent Adult Animals Child Child, Preschool Cross-Sectional Studies Developing Countries Humans New Guinea Plasmodium falciparum/*isolation & purification Plasmodium malariae/*isolation & purification Plasmodium vivax/*isolation & purification *Polymerase Chain Reaction/methods Sensitivity and Specificity
Pubmed
Web of science
Création de la notice
28/01/2008 12:49
Dernière modification de la notice
03/03/2018 18:49
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