CpG-A and CpG-B oligonucleotides differentially enhance human peptide-specific primary and memory CD8+ T-cell responses in vitro

Détails

ID Serval
serval:BIB_814DD173802B
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
CpG-A and CpG-B oligonucleotides differentially enhance human peptide-specific primary and memory CD8+ T-cell responses in vitro
Périodique
Blood
Auteur(s)
Rothenfusser  S., Hornung  V., Ayyoub  M., Britsch  S., Towarowski  A., Krug  A., Sarris  A., Lubenow  N., Speiser  D., Endres  S., Hartmann  G.
ISSN
0006-4971 (Print)
Statut éditorial
Publié
Date de publication
03/2004
Volume
103
Numéro
6
Pages
2162-9
Notes
In Vitro
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Mar 15
Résumé
Two distinct types of CpG oligodeoxynucleotide (ODN) have been identified that differ in their capacity to stimulate antigen-presenting cells: CpG-A induces high amounts of interferon-alpha (IFN-alpha) and IFN-beta in plasmacytoid dendritic cells (PDCs), whereas CpG-B induces PDC maturation and is a potent activator of B cells but stimulates only small amounts of IFN-alpha and IFN-beta. Here we examined the ability of these CpG ODNs to enhance peptide-specific CD8+ T-cell responses in human peripheral blood mononuclear cells (PBMCs). The frequency of influenza matrix-specific "memory" CD8+ T cells was increased by both types of CpG ODN, whereas the frequency of Melan-A specific "naive" CD8+ T cells increased on stimulation with CpG-B but not with CpG-A. The presence of PDCs in PBMCs was required for this CpG ODN-mediated effect. The expanded cells were cytotoxic and produced IFN- on peptide restimulation. Soluble factors induced by CpG-A but not CpG-B increased the granzyme-B content and cytotoxicity of established CD8+ T-cell clones, each of which was IFN-alpha/-beta dependent. In conclusion, CpG-B seems to be superior for priming CD8+ T-cell responses, and CpG-A selectively enhances memory CD8+ T-cell responses and induces cytotoxicity. These results demonstrate distinct functional properties of CpG-A and CpG-B with regard to CD8 T cells.
Mots-clé
Antigens, Neoplasm CD8-Positive T-Lymphocytes/cytology/*immunology/metabolism Cell Communication/immunology Cell Differentiation/immunology Cells, Cultured *CpG Islands Dendritic Cells/cytology/immunology Humans Immunologic Memory/*physiology Immunophenotyping Interferon Type II/metabolism Interferon-alpha/metabolism Interferon-beta/metabolism Neoplasm Proteins/immunology Oligodeoxyribonucleotides/*pharmacology Peptide Fragments/immunology T-Lymphocytes, Cytotoxic/cytology/immunology/metabolism Viral Matrix Proteins/immunology
Pubmed
Web of science
Création de la notice
28/01/2008 12:33
Dernière modification de la notice
03/03/2018 18:47
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