Endoplasmic reticulum accumulation of Kir6.2 without activation of ER stress response in islet cells from adult Sur1 knockout mice.

Détails

ID Serval
serval:BIB_814346F77481
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Endoplasmic reticulum accumulation of Kir6.2 without activation of ER stress response in islet cells from adult Sur1 knockout mice.
Périodique
Cell and Tissue Research
Auteur(s)
Marhfour I., Jonas J.C., Marchandise J., Lefevre A., Rahier J., Sempoux C., Guiot Y.
ISSN
1432-0878 (Electronic)
ISSN-L
0302-766X
Statut éditorial
Publié
Date de publication
2010
Peer-reviewed
Oui
Volume
340
Numéro
2
Pages
335-346
Langue
anglais
Notes
Publication types: Journal Article Publication Status: ppublish
Résumé
Trafficking of pancreatic K(ATP) channels to the plasma membrane critically depends on masking the endoplasmic reticulum (ER) retention signals of the SUR1 and Kir6.2 subunits upon their proper assembly into functional hetero-octamers. When expressed in the absence of the partner protein, each subunit might accumulate in the ER and trigger beta-cell ER stress and oxidative stress. To test this hypothesis, Kir6.2 localisation, ER ultra-structure and ER-stress- and oxidative-stress-response gene mRNA levels were evaluated in pancreatic endocrine cells from adult wild-type (WT) and Sur1 knockout (Sur1 ( -/- )) mice. As previously reported, Kir6.2 was mainly expressed on secretory granules and at the plasma membrane of WT islet cells. In contrast, like the ER chaperone calreticulin, Kir6.2 was primarily localised in the rough endoplasmic reticulum (RER) of Sur1 ( -/- ) islet cells. ER retention of Kir6.2 was demonstrated (electron microscopy) by a significant increase in the length and Kir6.2 density of RER in Sur1 ( -/- ) vs WT islet cells. Despite Kir6.2 retention in RER, Xbp1 mRNA splicing and mRNA levels of preproinsulin and ER-stress-response genes Bip, Edem and Gadd153 were similar in WT and Sur1 ( -/- ) islets. However, mRNA levels of the antioxidant enzymes Sod1, Sod2, Gpx2 and catalase were significantly up-regulated in Sur1 ( -/- ) islets. Sequestration of Kir6.2 in RER of Sur1 ( -/- ) islet cells is thus associated with an increase in RER length and mild oxidative stress without activation of the classical ER stress response.
Mots-clé
ATP-Binding Cassette Transporters/genetics, ATP-Binding Cassette Transporters/metabolism, Animals, Antibody Specificity/immunology, Calreticulin/metabolism, Endoplasmic Reticulum/genetics, Endoplasmic Reticulum/metabolism, Gene Expression Regulation, Islets of Langerhans/cytology, Islets of Langerhans/metabolism, Mice, Mice, Inbred C57BL, Mice, Knockout, Oxidative Stress/genetics, Potassium Channels, Inwardly Rectifying/deficiency, Potassium Channels, Inwardly Rectifying/genetics, Protein Transport, RNA, Messenger/genetics, RNA, Messenger/metabolism, Receptors, Drug/deficiency, Receptors, Drug/genetics, Stress, Physiological, Subcellular Fractions/metabolism, Sulfonylurea Receptors
Pubmed
Web of science
Création de la notice
29/01/2015 13:36
Dernière modification de la notice
03/03/2018 18:47
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