Rapid analysis of metabolites and drugs of abuse from urine samples by desorption electrospray ionization-mass spectrometry.

Details

Serval ID
serval:BIB_807EF2DBE206
Type
Article: article from journal or magazin.
Collection
Publications
Title
Rapid analysis of metabolites and drugs of abuse from urine samples by desorption electrospray ionization-mass spectrometry.
Journal
The Analyst
Author(s)
Kauppila T.J., Talaty N., Kuuranne T., Kotiaho T., Kostiainen R., Cooks R.G.
ISSN
0003-2654 (Print)
ISSN-L
0003-2654
Publication state
Published
Issued date
09/2007
Peer-reviewed
Oui
Volume
132
Number
9
Pages
868-875
Language
english
Notes
Publication types: Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S.
Publication Status: ppublish
Abstract
Urine samples obtained from drug abusers were screened for drugs of abuse and their metabolites using DESI-MS and the results obtained were compared to results obtained from GC-MS experiments. The detected analyte classes included amphetamines, opiates, cannabinoids and benzodiazepines. The compounds detected were codeine, morphine, oxymorphone, 11-nor-9-carboxy-Delta(9)-tetrahydrocannabinol, Delta(9)-tetrahydrocannabinol, alprazolam, temazepam, oxazepam, N-desmethyldiazepam (nordiazepam) and hydroxytemazepam. Identities of all the analytes were confirmed by tandem mass spectrometry, matching MS/MS spectra with authentic standard compounds. The concentrations of the analytes in the samples were obtained from semi-quantitative GC-MS studies and were in the range of 270-22,000 ng mL(-1). The analytes could be detected by DESI even after a hundred-fold dilution indicating that the sensitivity of DESI was more than adequate for this study. Selectivity in the DESI-MS measurements for different kinds of analytes could be increased further by optimizing the spray solvent composition: the use of an entirely aqueous solvent enhanced the signal of polar analytes, such as the benzodiazepines, whereas the use of a spray solvent with a high organic content increased the signal of less polar analytes, such as codeine and morphine.

Keywords
Gas Chromatography-Mass Spectrometry, Humans, Reproducibility of Results, Spectrometry, Mass, Electrospray Ionization/instrumentation, Spectrometry, Mass, Electrospray Ionization/methods, Street Drugs/urine, Substance Abuse Detection/instrumentation, Substance Abuse Detection/methods
Pubmed
Web of science
Create date
02/05/2017 14:05
Last modification date
20/08/2019 14:41
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