The efficiency of a novel delivery system (PEI600-Tat) in development of potent DNA vaccine using HPV16 E7 as a model antigen.
Details
Serval ID
serval:BIB_7FDD02856437
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
The efficiency of a novel delivery system (PEI600-Tat) in development of potent DNA vaccine using HPV16 E7 as a model antigen.
Journal
Drug Delivery
ISSN
1521-0464 (Electronic)
ISSN-L
1071-7544
Publication state
Published
Issued date
2009
Volume
16
Number
4
Pages
196-204
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Publication Status: ppublish
Abstract
DNA vaccination is a promising approach for inducing both humoral and cellular immune responses. The mode of plasmid DNA delivery is critical to make progress in DNA vaccination. Using human papillomavirus type 16 E7 as a model antigen, this study evaluated the effect of peptide-polymer hybrid including PEI600-Tat conjugate as a novel gene delivery system on the potency of antigen-specific immunity in mice model. At ratio of 10:50 PEI-Tat/E7DNA (w/w), both humoral and cellular immune responses were significantly enhanced as compared with E7DNA construct and induced Th1 response. Therefore, this new delivery system could have promising applications in gene therapy.
Keywords
Animals, Antibody Formation/drug effects, Avidin/chemistry, Drug Carriers/administration & dosage, Drug Carriers/chemistry, Drug Delivery Systems, Female, Humans, Immunity, Cellular/drug effects, Mice, Mice, Inbred C57BL, Oncogene Proteins, Viral/genetics, Oncogene Proteins, Viral/immunology, Papillomavirus E7 Proteins, Polyethyleneimine/chemistry, Transfection, Vaccines, DNA/administration & dosage, Vaccines, DNA/chemistry
Pubmed
Web of science
Create date
24/09/2010 9:12
Last modification date
20/08/2019 15:40
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