Quick and reliable assessment of chronological life span in yeast cell populations by flow cytometry.

Details

Serval ID
serval:BIB_7F01270D8B86
Type
Article: article from journal or magazin.
Collection
Publications
Title
Quick and reliable assessment of chronological life span in yeast cell populations by flow cytometry.
Journal
Mechanisms of ageing and development
Author(s)
Ocampo A., Barrientos A.
ISSN
1872-6216 (Electronic)
ISSN-L
0047-6374
Publication state
Published
Issued date
2011
Peer-reviewed
Oui
Volume
132
Number
6-7
Pages
315-323
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
Yeast chronological life span (CLS) is defined as the capacity of stationary cultures to maintain viability over time, thus mimicking the situation of post-mitotic cells in multicellular organisms. Cellular viability is typically determined by using the colony formation unit assay (CFU). CFU counting is simple but laborious and does not accommodate large-scale experiments. Importantly, viability is determined on the basis of the cell's ability to divide and form a colony, probably not the ideal parameter when studying post-mitotic cellular ageing. This study describes the optimization and validation of a method based on the flow cytometric monitoring of propidium iodide (PI) uptake for assessing yeast cell death during CLS. The optimized protocol is quick, reliable, reproducible and can accommodate high-throughput studies. The method was validated by determining CLS of several strains used in yeast ageing research and by evaluating the effect of genetic disturbances known to extend or reduce yeast chronological life span.
Keywords
Flow Cytometry/methods, Saccharomyces cerevisiae/cytology, Saccharomyces cerevisiae/metabolism
Pubmed
Web of science
Create date
14/08/2018 9:47
Last modification date
20/08/2019 14:39
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