Apolipoprotein(a) kringle 4-containing fragments in human urine. Relationship to plasma levels of lipoprotein(a).

Details

Serval ID
serval:BIB_7EC6C8779A9F
Type
Article: article from journal or magazin.
Collection
Publications
Title
Apolipoprotein(a) kringle 4-containing fragments in human urine. Relationship to plasma levels of lipoprotein(a).
Journal
Journal of Clinical Investigation
Author(s)
Mooser V., Seabra M.C., Abedin M., Landschulz K.T., Marcovina S., Hobbs H.H.
ISSN
0021-9738 (Print)
ISSN-L
0021-9738
Publication state
Published
Issued date
1996
Volume
97
Number
3
Pages
858-864
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't
Abstract
Apo(a) is a large glycoprotein of unknown function that circulates in plasma as part of lipoprotein(a). Apo(a) is structurally related to plasminogen and contains at least 10 kringle (K)4 repeats (type 1-10), a K5 repeat and sequences similar to the protease domain of plasminogen. Plasminogen generates two biologically active peptides: plasmin and angiostatin, a kringle-containing peptide. As a first step in determining if apo(a) generates a similar kringle-containing peptide, human urine was immunologically examined. Fragments ranging in size from 85 to 215 kD were immunodetected using antibodies directed against epitopes in the K4-type 2 repeat, but not the K4-type 9 repeat or protease domain, NH2-terminal sequence analysis revealed sequences specific for the K4-type 1 repeat, confirming that the fragments are from the NH2 terminus of the K4 array. The amount of urinary apo(a) rose in proportion to the plasma lipoprotein(a) concentration. Even individuals with trace to no apo(a) in plasma had immunodetectable apo(a) fragments in their urine. Intravenous administration of the human urinary apo(a) into mice resulted in the urine. These findings suggest that the apo(a) fragments found in urine are formed extrarenally and then excreted by the kidney.
Keywords
Amino Acid Sequence, Animals, Apolipoproteins/blood, Apolipoproteins/chemistry, Apoprotein(a), Humans, Immunoblotting, Kidney/metabolism, Kringles, Lipoprotein(a), Mice, Molecular Sequence Data, Peptide Fragments/chemistry, Peptide Fragments/immunology, Sequence Analysis
Pubmed
Web of science
Open Access
Yes
Create date
13/10/2013 21:33
Last modification date
20/08/2019 14:39
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