Measuring glucose cerebral metabolism in the healthy mouse using hyperpolarized (13)C magnetic resonance.

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Etat: Serval
Version: Final published version
ID Serval
serval:BIB_7E6E52E2D4F7
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Measuring glucose cerebral metabolism in the healthy mouse using hyperpolarized (13)C magnetic resonance.
Périodique
Scientific reports
Auteur(s)
Mishkovsky M., Anderson B., Karlsson M., Lerche M.H., Sherry A.D., Gruetter R., Kovacs Z., Comment A.
ISSN
2045-2322 (Electronic)
ISSN-L
2045-2322
Statut éditorial
Publié
Date de publication
15/09/2017
Peer-reviewed
Oui
Volume
7
Numéro
1
Pages
11719
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: epublish
Résumé
The mammalian brain relies primarily on glucose as a fuel to meet its high metabolic demand. Among the various techniques used to study cerebral metabolism, (13)C magnetic resonance spectroscopy (MRS) allows following the fate of (13)C-enriched substrates through metabolic pathways. We herein demonstrate that it is possible to measure cerebral glucose metabolism in vivo with sub-second time resolution using hyperpolarized (13)C MRS. In particular, the dynamic (13)C-labeling of pyruvate and lactate formed from (13)C-glucose was observed in real time. An ad-hoc synthesis to produce [2,3,4,6,6-(2)H5, 3,4-(13)C2]-D-glucose was developed to improve the (13)C signal-to-noise ratio as compared to experiments performed following [U-(2)H7, U-(13)C]-D-glucose injections. The main advantage of only labeling C3 and C4 positions is the absence of (13)C-(13)C coupling in all downstream metabolic products after glucose is split into 3-carbon intermediates by aldolase. This unique method allows direct detection of glycolysis in vivo in the healthy brain in a noninvasive manner.

Pubmed
Web of science
Open Access
Oui
Création de la notice
05/10/2017 10:33
Dernière modification de la notice
08/05/2019 21:00
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