Topological locking restrains replication fork reversal.

Details

Serval ID
serval:BIB_7D502D13B119
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Topological locking restrains replication fork reversal.
Journal
Proceedings of the National Academy of Sciences of the United States of America
Author(s)
Fierro-Fernández M., Hernández P., Krimer D.B., Stasiak A., Schvartzman J.B.
ISSN
0027-8424[print], 0027-8424[linking]
Publication state
Published
Issued date
01/2007
Peer-reviewed
Oui
Volume
104
Number
5
Pages
1500-1505
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
Two-dimensional agarose gel electrophoresis, psoralen cross-linking, and electron microscopy were used to study the effects of positive supercoiling on fork reversal in isolated replication intermediates of bacterial DNA plasmids. The results obtained demonstrate that the formation of Holliday-like junctions at both forks of a replication bubble creates a topological constraint that prevents further regression of the forks. We propose that this topological locking of replication intermediates provides a biological safety mechanism that protects DNA molecules against extensive fork reversals.
Keywords
Blotting, Southern, Cross-Linking Reagents/pharmacology, DNA/chemistry, DNA Repair, DNA, Bacterial/chemistry, DNA, Superhelical/chemistry, Electrophoresis, Gel, Two-Dimensional/methods, Escherichia coli/metabolism, Ficusin/chemistry, Intercalating Agents/pharmacology, Microscopy, Electron/methods, Nucleic Acid Conformation, Nucleic Acid Hybridization, Plasmids/metabolism, Temperature
Pubmed
Web of science
Open Access
Yes
Create date
24/01/2008 10:36
Last modification date
20/08/2019 14:38
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