An agarose matrix facilitates sectioning of tissue microarray blocks.

Détails

ID Serval
serval:BIB_7CEEDFCE46F6
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
An agarose matrix facilitates sectioning of tissue microarray blocks.
Périodique
Journal of Histochemistry and Cytochemistry
Auteur(s)
Yan P., Seelentag W., Bachmann A., Bosman F.T.
ISSN
0022-1554
Statut éditorial
Publié
Date de publication
2007
Peer-reviewed
Oui
Volume
55
Numéro
1
Pages
21-24
Langue
anglais
Résumé
Tissue microarray (TMA) is a powerful, high-throughput technique for in situ investigation of biomarkers in many tissue samples in a paraffin block by immunohistochemistry or fluorescence in situ hybridization (FISH), and has rapidly become the standard in marker studies. One of the difficult steps in the procedure is the sectioning of array blocks and mounting of sections using special slides and/or adhesive-coated tape, which demands specific experience and is time-consuming. We report an arraying method that allows melting of the receiving paraffin block and subsequent sectioning like any ordinary paraffin-embedded tissue block. The major difference from the standard microarray technique is the use of an agarose matrix in the recipient block. The agarose matrix allows melting of the paraffin without disturbing the array, resulting in perfect integration of the tissue cores. The agarose-paraffin TMA blocks limit tissue core loss during cutting, mounting, or immunohistochemical or FISH staining and better maintains the array.
Mots-clé
Hepatocytes, Immunohistochemistry, In Situ Hybridization, Fluorescence, Keratin-6, Keratin-7, Microtomy, Paraffin, Receptor, Epidermal Growth Factor, Sepharose, Serum Amyloid A Protein, Tissue Array Analysis, Tissue Embedding
Pubmed
Web of science
Création de la notice
29/01/2008 19:33
Dernière modification de la notice
03/03/2018 18:38
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