Antigenic crossreactivity of the alpha subunit of complement component C8 with the cysteine-rich domain shared by complement component C9 and low density lipoprotein receptor
Details
Serval ID
serval:BIB_7AC909A1305B
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Antigenic crossreactivity of the alpha subunit of complement component C8 with the cysteine-rich domain shared by complement component C9 and low density lipoprotein receptor
Journal
Proceedings of the National Academy of Sciences of the United States of America
ISSN
0027-8424 (Print)
Publication state
Published
Issued date
06/1986
Volume
83
Number
12
Pages
4223-7
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Jun
Research Support, Non-U.S. Gov't --- Old month value: Jun
Abstract
Complement component C9 contains two distinct cysteine-rich domains exhibiting high sequence resemblance to a domain present in the low density lipoprotein (LDL) receptor and epidermal growth factor precursor, respectively. Antibodies were raised against a peptide corresponding to the most conserved region of the LDL receptor/C9-homology segment. The antibodies were shown by immunoblotting to bind specifically to C9 but also to crossreact with C8 alpha, the alpha subunit of complement component C8. Moreover, a monoclonal antibody to a neoantigen present in polymerized C9 bound to an epitope exposed on C8 within the C5b-8 complex but buried in monomeric C8, suggesting that C8 and C9 undergo similar conformational changes during membrane-attack-complex assembly. Isolated C8 alpha-gamma exhibited the propensity to polymerize in the presence of Zn2+ and urea, as already demonstrated for C9. These data indicate that C8 alpha is closely related, both structurally and functionally, to C9.
Keywords
Complement C8/*immunology
Complement C9/*immunology
Complement Membrane Attack Complex
Complement System Proteins/immunology
Cross Reactions
Cysteine
Epitopes
Humans
Macromolecular Substances
Oligopeptides/chemical synthesis/immunology
Receptors, LDL/*immunology
Pubmed
Web of science
Open Access
Yes
Create date
24/01/2008 16:18
Last modification date
20/08/2019 15:36