Bombesin does not stimulate pepsinogen release in isolated gastric chief cells
Details
Serval ID
serval:BIB_7AB2A0EE9368
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Bombesin does not stimulate pepsinogen release in isolated gastric chief cells
Journal
Peptides
ISSN
0196-9781 (Print)
Publication state
Published
Issued date
1995
Volume
16
Number
3
Pages
383-8
Notes
In Vitro
Journal Article
Journal Article
Abstract
Bombesin (BN)-related peptides, such as gastrin-releasing peptide (GRP), have been shown in vivo to stimulate release of pepsinogen. However, whether this is due to a direct interaction with chief cells is not clear. To clarify this we prepared isolated chief cells (> 90% pure) from guinea pig stomach. BN, GRP, or neuromedin B (NMB), at concentrations up to 1 microM, did not stimulate pepsinogen release or affect the stimulation caused by vasoactive intestinal peptide (VIP) (100 nM) or CCK-8 (10 nM), respectively. In addition, BN, GRP, or NMB at a concentration of 1 microM did not increase cAMP nor did they alter the increase in cAMP caused by VIP or secretin. BN (1 microM) did not alter basal cytosolic calcium [Ca2+]i or affect the increase in [Ca2+]i caused by CCK-8 (1 microM). Furthermore, BN, GRP, or NMB at a concentration of 1 microM did not increase the generation of inositol phosphates (IP) or alter the increase in [3H]IP1, [3H]IP2, or [3H]IP3, caused by CCK-8 (1 microM) or carbachol (1 mM). Binding studies demonstrated no saturable binding of either [125I][Tyr4]BN or [125I][D-Tyr0]NMB using experimental conditions where binding with other peptide ligands to other receptors on chief cells is seen. We conclude that BN-related peptides do not interact directly with specific receptors on chief cells to stimulate or alter stimulated pepsinogen secretion, increase the breakdown of inositol phosphates, or alter [Ca2+]i or cAMP.
Keywords
Analysis of Variance
Animals
Bombesin/analogs & derivatives/metabolism/*pharmacology
Gastric Mucosa/*drug effects/secretion
Guinea Pigs
Male
Pepsinogens/*secretion
Radioligand Assay
Receptors, Bombesin/*metabolism
Stimulation, Chemical
Pubmed
Web of science
Create date
25/01/2008 16:58
Last modification date
20/08/2019 15:36