Hepatitis B virus-related insertional mutagenesis implicates SERCA1 gene in the control of apoptosis

Détails

ID Serval
serval:BIB_79F4881F0A62
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Hepatitis B virus-related insertional mutagenesis implicates SERCA1 gene in the control of apoptosis
Périodique
Oncogene
Auteur(s)
Chami  M., Gozuacik  D., Saigo  K., Capiod  T., Falson  P., Lecoeur  H., Urashima  T., Beckmann  J., Gougeon  M. L., Claret  M., le Maire  M., Brechot  C., Paterlini-Brechot  P.
ISSN
0950-9232 (Print)
Statut éditorial
Publié
Date de publication
06/2000
Volume
19
Numéro
25
Pages
2877-86
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Jun 8
Résumé
We have used the Hepatitis B Virus DNA genome as a probe to identify genes clonally mutated in vivo, in human liver cancers. In a tumor, HBV-DNA was found to be integrated into the gene encoding Sarco/Endoplasmic Reticulum Calcium ATPase (SERCA), which pumps calcium, an important intracellular messenger for cell viability and growth, from the cytosol to the endoplasmic reticulum. The HBV X gene promoter cis-activates chimeric HBV X/SERCA1 transcripts, with splicing of SERCA1 exon 11, encoding C-terminally truncated SERCA1 proteins. Two chimeric HBV X/SERCA1 proteins accumulate in the tumor and form dimers. In vitro analyses have demonstrated that these proteins localize to the ER, determine its calcium depletion and induce cell death. We have also shown that these biological effects are related to expression of the SERCA, rather than of the viral moiety. This report involves for the first time the expression of mutated SERCA proteins in vivo in a tumor cell proliferation and in vitro in the control of cell viability. Oncogene (2000).
Mots-clé
Aged Apoptosis/*genetics Calcium-Transporting ATPases/*genetics/metabolism Dimerization Hepatitis B virus/*physiology Humans Mutagenesis, Insertional/*genetics RNA, Messenger/genetics Recombinant Fusion Proteins/genetics/metabolism Sarcoplasmic Reticulum/enzymology Tumor Cells, Cultured Virus Integration
Pubmed
Web of science
Open Access
Oui
Création de la notice
25/01/2008 17:18
Dernière modification de la notice
08/05/2019 20:45
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