Thermolysin treatment: a new method for dermo-epidermal separation.

Détails

ID Serval
serval:BIB_79B94BCE580E
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Thermolysin treatment: a new method for dermo-epidermal separation.
Périodique
Journal of Investigative Dermatology
Auteur(s)
Walzer C., Benathan M., Frenk E.
ISSN
0022-202X
Statut éditorial
Publié
Date de publication
01/1989
Peer-reviewed
Oui
Volume
92
Numéro
1
Pages
78-81
Langue
anglais
Notes
Publication types: Comparative Study ; Journal Article - Publication Status: ppublish
Résumé
The epidermis of superficial human skin samples could easily be separated from the dermis following incubation at +4 degrees C for 1 h in a solution containing 250-500 micrograms/ml thermolysin, a proteolytic enzyme hitherto mostly used for protein analysis. Light and electron microscopy revealed that the dermo-epidermal separation occurred at the basement membrane between the sites of bullous pemphigoid antigen and laminin and that the hemidesmosomes were selectively disrupted. The cohesion and morphology of the separated epidermis as well as the immunologic parameters investigated were not altered by this procedure. The clear cut dermo-epidermal separation produced by thermolysin treatment differed from the separation obtained with trypsin, which predominantly occurred between basal and suprabasal cells by disruption of desmosomes.
Mots-clé
Adult, Aged, Basement Membrane, Child, Child, Preschool, Epidermis, Female, Histological Techniques, Humans, Microscopy, Electron, Skin, Thermolysin, Trypsin
Pubmed
Web of science
Open Access
Oui
Création de la notice
28/01/2008 9:20
Dernière modification de la notice
08/05/2019 20:44
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