Chromosomes analysis of 28 shrews belonging to the genus Crocidura from West Africa allowed the detection of five new karyotypes: C. wimmeri (2n = 50, NF = 84), C. cf. gracilipes (56/86), C. cf. nimbae (46/68), and C. cf. planiceps (44/72). Among the individuals identified morphologically as C. poensis (52/70) a distinct species was recognized on the basis of its chromosome complement, C. nigeriae (50/76). The karyotype of C. occidentalis was confirmed with the study of another subspecies, C. occidentalis manni (50/66). An identical chromosome set characterizes C. odorata giffardi and provides evidence for its relationship with C. occidentalis.