Vitellogenin in Xenopus laevis is encoded in a small family of genes.

Détails

ID Serval
serval:BIB_774EF17EF382
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Vitellogenin in Xenopus laevis is encoded in a small family of genes.
Périodique
Cell
Auteur(s)
Wahli W., Dawid I.B., Wyler T., Jaggi R.B., Weber R., Ryffel G.U.
ISSN
0092-8674[print], 0092-8674[linking]
Statut éditorial
Publié
Date de publication
03/1979
Volume
16
Numéro
3
Pages
535-549
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Résumé
Vitellogenin, the yolk protein precursor, is produced in X. laevis liver from a 6.3 kilobase (kb) mRNA. Sequences of this mRNA have been transcribed into cDNA and cloned in E. coli. Some properties of 21 of these cloned DNAs, ranging in size from 1 to 3.7 kb, have been reported by Wahli et al. (1978b). This paper reports restriction endonuclease mapping, cross hybridization, heteroduplex mapping in the electron microscope and heteroduplex melting experiments with these DNAs. We conclude that the cloned DNAs fall into two main groups of sequences which differ from each other in approximately 20% of their nucleotides. Each main group contains two subgroups which differ from each other by about 5% sequence divergence. By hybridizing cloned DNAs with restricted genomic DNA, we showed that sequences corresponding to all four sequence groups are present in a single animal. Furthermore, we have obtained tentative evidence for the presence of large intervening sequences in genomic vitellogenin DNA. Analysis of R loop molecules demonstrated that all four sequences are present in the vitellogenin mRNA population purified from individual animals. While some alternate explanations are not entirely excluded, we suggest that vitellogenin is encoded by a small family of related genes in Xenopus.
Mots-clé
Animals, Base Sequence, Genes, Lipoproteins/genetics, Nucleic Acid Hybridization, Poly A/analysis, Polymorphism, Genetic, RNA, Messenger/analysis, Vitellogenins/genetics, Xenopus/genetics
Pubmed
Web of science
Création de la notice
24/01/2008 17:04
Dernière modification de la notice
03/03/2018 18:27
Données d'usage