Vaccinia virus produces late mRNAs by discontinuous synthesis.

Détails

ID Serval
serval:BIB_77375F9948BE
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Vaccinia virus produces late mRNAs by discontinuous synthesis.
Périodique
Cell
Auteur(s)
Bertholet C., Van Meir E., ten Heggeler-Bordier B., Wittek R.
ISSN
0092-8674[print], 0092-8674[linking]
Statut éditorial
Publié
Date de publication
07/1987
Volume
50
Numéro
2
Pages
153-162
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
We describe the unusual structure of a vaccinia virus late mRNA. In these molecules, the protein-coding sequences of a major late structural polypeptide are preceded by long leader RNAs, which in some cases are thousands of nucleotides long. These sequences map to different regions of the viral genome and in one instance are separated from the late gene by more than 100 kb of DNA. Moreover, the leader sequences map either upstream or downstream of the late gene, are transcribed from either DNA strand, and are fused to the late gene coding sequence via a poly(A) stretch. This demonstrates that vaccinia virus produces late mRNAs by tagging the protein-coding sequences onto the 3' end of other RNAs.
Mots-clé
Base Sequence, Cloning, Molecular, DNA/metabolism, DNA Restriction Enzymes, DNA, Viral/genetics, Genes, Viral, Hela Cells/metabolism, Humans, Molecular Weight, Nucleic Acid Hybridization, RNA, Messenger/biosynthesis, RNA, Messenger/genetics, Vaccinia virus/genetics
Pubmed
Web of science
Création de la notice
24/01/2008 11:43
Dernière modification de la notice
03/03/2018 18:27
Données d'usage