Minocycline-induced activation of tetracycline-responsive promoter.

Détails

ID Serval
serval:BIB_7609B9070C7F
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Minocycline-induced activation of tetracycline-responsive promoter.
Périodique
Neuroscience Letters
Auteur(s)
Chtarto A., Tenenbaum L., Velu T., Brotchi J., Levivier M., Blum D.
ISSN
0304-3940 (Print)
ISSN-L
0304-3940
Statut éditorial
Publié
Date de publication
2003
Peer-reviewed
Oui
Volume
352
Numéro
3
Pages
155-158
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
Minocycline has been suggested to be an anti-apoptotic compound and an anti-inflammatory agent in various models of neurodegeneration. In the present study, using a stable cell line expressing green fluorescent protein under the control of a tetracycline-responsive promoter, we demonstrate that minocycline is able to promote tetracycline-controlled gene expression although it needs longer time and higher concentration to reach the effect obtained with the classical inducer doxycycline. Furthermore, the extinction of the system after antibiotics removal is faster when using minocycline. Interestingly, minocycline displays lower cytotoxicity than doxycycline. It is thus tempting to speculate that combining the intrinsic neuroprotective activity of minocycline with its ability to induce tetracycline-regulatable promoters would be greatly beneficial for neuroprotective/neurorestaurative gene therapy.
Mots-clé
Cell Line, Tumor, Cell Survival/drug effects, Cell Survival/physiology, Dose-Response Relationship, Drug, Gene Expression Regulation/drug effects, Humans, Minocycline/pharmacology, Promoter Regions, Genetic/drug effects, Tetracycline/pharmacology
Pubmed
Web of science
Création de la notice
20/01/2008 18:35
Dernière modification de la notice
03/03/2018 18:24
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