Neutralization of Macrophage Migration Inhibitory Factor (MIF) by Fully Human Antibodies Correlates with Their Specificity for the β-Sheet Structure of MIF.

Détails

ID Serval
serval:BIB_73FC2C8192D5
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Neutralization of Macrophage Migration Inhibitory Factor (MIF) by Fully Human Antibodies Correlates with Their Specificity for the β-Sheet Structure of MIF.
Périodique
Journal of Biological Chemistry
Auteur(s)
Kerschbaumer R.J., Rieger M., Völkel D., Le Roy D., Roger T., Garbaraviciene J., Boehncke W.H., Müllberg J., Hoet R.M., Wood C.R., Antoine G., Thiele M., Savidis-Dacho H., Dockal M., Ehrlich H., Calandra T., Scheiflinger F.
ISSN
1083-351X (Electronic)
ISSN-L
0021-9258
Statut éditorial
Publié
Date de publication
2012
Volume
287
Numéro
10
Pages
7446-7455
Langue
anglais
Notes
Publication types: Journal ArticlePublication Status: ppublish
Résumé
The macrophage migration inhibitory factor (MIF) is a proinflammatory cytokine that recently emerged as an attractive therapeutic target for a variety of diseases. A diverse panel of fully human anti-MIF antibodies was generated by selection from a phage display library and extensively analyzed in vitro. Epitope mapping studies identified antibodies specific for linear as well as structural epitopes. Experimental animal studies revealed that only those antibodies binding epitopes within amino acids 50-68 or 86-102 of the MIF molecule exerted protective effects in models of sepsis or contact hypersensitivity. Within the MIF protein, these two binding regions form a β-sheet structure that includes the MIF oxidoreductase motif. We therefore conclude that this β-sheet structure is a crucial region for MIF activity and a promising target for anti-MIF antibody therapy.
Pubmed
Web of science
Open Access
Oui
Création de la notice
01/04/2012 14:43
Dernière modification de la notice
08/05/2019 20:26
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