Neuron-associated class III beta-tubulin isotype, microtubule-associated protein 2, and synaptophysin in human retinoblastomas in situ. Further immunohistochemical observations on the Flexner-Wintersteiner rosettes

Détails

ID Serval
serval:BIB_73C90784C15D
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Neuron-associated class III beta-tubulin isotype, microtubule-associated protein 2, and synaptophysin in human retinoblastomas in situ. Further immunohistochemical observations on the Flexner-Wintersteiner rosettes
Périodique
Laboratory Investigation
Auteur(s)
Katsetos  C. D., Herman  M. M., Frankfurter  A., Uffer  S., Perentes  E., Rubinstein  L. J.
ISSN
0023-6837 (Print)
Statut éditorial
Publié
Date de publication
01/1991
Volume
64
Numéro
1
Pages
45-54
Notes
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S. --- Old month value: Jan
Résumé
We studied by immunohistochemistry 26 retinoblastomas in situ using monoclonal antibodies specific for the neuron-associated class III beta-tubulin isotype (h beta 4), microtubule-associated protein 2 (MAP2), and synaptophysin. Anti-h beta 4 and anti-MAP2 immunostaining was consistently obtained in the Flexner-Wintersteiner rosettes, in fleurettes, in Homer Wright (neuroblastic) rosettes, and also variably among poorly differentiated tumor cells. A similar pattern of antisynaptophysin immunopositivity was seen, but was especially pronounced in the adluminal borders of cells forming the Flexner-Wintersteiner rosettes. The demonstration of h beta 4, MAP2, and synaptophysin epitopes in poorly differentiated and maturing neoplastic phenotypes in retinoblastomas attests to the neuronal character of this embryonal tumor. Immunoreactivity toward h beta 4 and MAP2 epitopes by poorly differentiated neoplastic cells may indicate early neuronal commitment in retinoblastoma. The consistent immunostaining of Flexner-Wintersteiner rosettes with monoclonal antibodies to h beta 4 and MAP2 is in keeping with the previous ultrastructural documentation of microtubules with a neuronal-like spatial organization present in the cells of these structures.
Mots-clé
Adult Cell Differentiation Epitopes Eye Neoplasms/*metabolism/pathology Glial Fibrillary Acidic Protein/metabolism Humans Immunohistochemistry Infant, Newborn Male Membrane Proteins/immunology/*metabolism Microtubule-Associated Proteins/immunology/*metabolism Middle Aged Neurons/*metabolism Retina/cytology/metabolism Retinoblastoma/*metabolism/pathology Staining and Labeling Synaptophysin Tubulin/immunology/*metabolism
Pubmed
Web of science
Création de la notice
28/01/2008 14:02
Dernière modification de la notice
03/03/2018 18:20
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