Microfluidic systems in proteomics.

Details

Serval ID
serval:BIB_73C3E38CB56B
Type
Article: article from journal or magazin.
Publication sub-type
Review (review): journal as complete as possible of one specific subject, written based on exhaustive analyses from published work.
Collection
Publications
Title
Microfluidic systems in proteomics.
Journal
Electrophoresis
Author(s)
Lion N., Rohner T.C., Dayon L., Arnaud I.L., Damoc E., Youhnovski N., Wu Z.Y., Roussel C., Josserand J., Jensen H., Rossier J.S., Przybylski M., Girault H.H.
ISSN
0173-0835 (Print)
ISSN-L
0173-0835
Publication state
Published
Issued date
2003
Peer-reviewed
Oui
Volume
24
Number
21
Pages
3533-3562
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't ; Review Publication Status: ppublish
Abstract
We present the state-of-the-art in miniaturized sample preparation, immunoassays, one-dimensional and multidimensional analyte separations, and coupling of microdevices with electrospray ionization-mass spectrometry. Hyphenation of these different techniques and their relevance to proteomics will be discussed. In particular, we will show that analytical performances of microfluidic analytical systems are already close to fulfill the requirements for proteomics, and that miniaturization results at the same time in a dramatic increase in analysis throughput. Throughout this review, some examples of analytical operations that cannot be achieved without microfluidics will be emphasized. Finally, conditions for the spreading of microanalytical systems in routine proteomic labs will be discussed.
Keywords
Electrophoresis/methods, Enzymes/analysis, Enzymes/metabolism, Microfluidics/methods, Miniaturization/methods, Proteome
Pubmed
Web of science
Create date
09/11/2014 22:51
Last modification date
20/08/2019 14:31
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