Induction of spine growth and synapse formation by regulation of the spine actin cytoskeleton

Détails

ID Serval
serval:BIB_722E71E40A49
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Induction of spine growth and synapse formation by regulation of the spine actin cytoskeleton
Périodique
Neuron
Auteur(s)
Zito  K., Knott  G., Shepherd  G. M., Shenolikar  S., Svoboda  K.
ISSN
0896-6273 (Print)
Statut éditorial
Publié
Date de publication
10/2004
Volume
44
Numéro
2
Pages
321-34
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Oct 14
Résumé
We explored the relationship between regulation of the spine actin cytoskeleton, spine morphogenesis, and synapse formation by manipulating expression of the actin binding protein NrbI and its deletion mutants. In pyramidal neurons of cultured rat hippocampal slices, NrbI is concentrated in dendritic spines by binding to the actin cytoskeleton. Expression of one NrbI deletion mutant, containing the actin binding domain, dramatically increased the density and length of dendritic spines with synapses. This hyperspinogenesis was accompanied by enhanced actin polymerization and spine motility. Synaptic strengths were reduced to compensate for extra synapses, keeping total synaptic input per neuron constant. Our data support a model in which synapse formation is promoted by actin-powered motility.
Mots-clé
Actins/*physiology Amino Acid Sequence Animals Cytoskeleton/*physiology Dendrites/*physiology/ultrastructure Microfilament Proteins/*genetics/metabolism Microscopy, Electron Models, Neurological Molecular Sequence Data Mutation Nerve Tissue Proteins/*genetics/metabolism Neuronal Plasticity Patch-Clamp Techniques Pyramidal Cells/*cytology Rats Sequence Homology Synapses/physiology/ultrastructure Transfection
Pubmed
Web of science
Création de la notice
24/01/2008 15:26
Dernière modification de la notice
03/03/2018 18:16
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