Fluorescence detection of symmetric GroEL14(GroES7)2 heterooligomers involved in protein release during the chaperonin cycle.

Détails

ID Serval
serval:BIB_71467E6F2D81
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Fluorescence detection of symmetric GroEL14(GroES7)2 heterooligomers involved in protein release during the chaperonin cycle.
Périodique
Journal of Biological Chemistry
Auteur(s)
Török Z., Vigh L., Goloubinoff P.
ISSN
0021-9258 (Print)
ISSN-L
0021-9258
Statut éditorial
Publié
Date de publication
1996
Volume
271
Numéro
27
Pages
16180-16186
Langue
anglais
Résumé
The GroEL14 chaperonin from Escherichia coli was labeled with 5-((((2-iodoacetyl)amino)ethyl)amino)naphthalene-1-sulfonic acid (I-AEDANS), a hydrophobic probe whose fluorescent emission is sensitive to structural changes within the protein. Increasing concentrations of ATP or adenylyl imidodiphosphate but not ADP caused two successive GroES7-dependent changes in the fluorescence intensity of AEDANS-GroEL14, corresponding to the sequential binding of two GroES7 heptamers and the formation of two types of chaperonin heterooligomers, GroEL14GroES7 and GroEL14(GroES7)2. The binding of thermally denatured malate dehydrogenase (MDH) caused a specific increase in fluorescence intensity of AEDANS-GroEL14 that allowed the direct measurement in solution at equilibrium of ATP- and GroES7-dependent protein release from the chaperonin. Structure/function analysis during the generation of ATP from ADP indicated the following sequence of events: 1) ADP-stabilized MDH-GroEL14GroES7 particles bind newly formed ATP. 2) MDH-GroEL14GroES7 particles bind a second GroES7. 3) MDH-GroEL14(GroES7)2 particles productively release MDH. 4) Released MDH completes folding. Therefore, the symmetrical GroEL14(GroES7)2 heterooligomer is an intermediate after the formation of which the protein substrate is productively released during the chaperonin-mediated protein folding cycle.
Mots-clé
Adenosine Diphosphate/pharmacology, Adenosine Triphosphate/metabolism, Adenosine Triphosphate/pharmacology, Adenylyl Imidodiphosphate/pharmacology, Chaperonin 10/analysis, Chaperonin 10/chemistry, Chaperonin 60/analysis, Chaperonin 60/chemistry, Fluorescent Dyes, Kinetics, Macromolecular Substances, Magnesium/pharmacology, Malate Dehydrogenase/metabolism, Models, Structural, Protein Folding, Protein Multimerization, Spectrometry, Fluorescence/methods
Pubmed
Web of science
Création de la notice
24/01/2008 21:02
Dernière modification de la notice
20/08/2019 15:29
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