Lactate turnover in rat glioma measured by in vivo nuclear magnetic resonance spectroscopy.

Details

Serval ID
serval:BIB_7047CDD6C49E
Type
Article: article from journal or magazin.
Collection
Publications
Title
Lactate turnover in rat glioma measured by in vivo nuclear magnetic resonance spectroscopy.
Journal
Cancer Research
Author(s)
Terpstra M., Gruetter R., High W.B., Mescher M., DelaBarre L., Merkle H., Garwood M.
ISSN
0008-5472 (Print)
ISSN-L
0008-5472
Publication state
Published
Issued date
1998
Volume
58
Number
22
Pages
5083-5088
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, P.H.S.Publication Status: ppublish
Abstract
Elevated tissue lactate concentrations typically found in tumors can be measured by in vivo nuclear magnetic resonance (NMR) spectroscopy. In this study, lactate turnover in rat C6 glioma was determined from in vivo 1H NMR measurements of [3-13C]lactate buildup during steady-state hyperglycemia with [1-13C]glucose. With this tumor model, a narrow range of values was observed for the first-order rate constant that describes lactate efflux, k2 = 0.043 +/- 0.007 (n = 12) SD min-1. For individual animals, the standard error in k2 was small (< 18%), which indicated that the NMR data fit the kinetic model well. Lactate measurements before and after infusing [1-13C]glucose showed that the majority of the tumor lactate pool was metabolically active. Signals from 13C-labeled glutamate in tumors were at least 10-fold smaller than the [3-13C]lactate signal, whereas spectra of the contralateral hemispheres revealed the expected labeling of [4-13C]glutamate, as well as [2-13C] and [3-13C]glutamate, which indicates that label cycled through the tricarboxylic acid cycle in the brain tissue. Lack of significant 13C labeling of glutamate was consistent with low respiratory metabolism in this glioma. It is concluded that lactate in rat C6 glioma is actively turning over and that the kinetics of lactate efflux can be quantified noninvasively by 1H NMR detection of 13C label. This noninvasive NMR approach may offer a valuable tool to help evaluate tumor growth and metabolic responsiveness to therapies.
Keywords
Animals, Brain Neoplasms/metabolism, Carbon Isotopes, Glioma/metabolism, Glucose/administration & dosage, Glucose/metabolism, Glutamic Acid, Glycolysis, Lactic Acid/metabolism, Magnetic Resonance Spectroscopy, Rats
Pubmed
Web of science
Create date
04/08/2010 15:28
Last modification date
20/08/2019 14:29
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