An axial domain of HOM/Hox gene expression is formed by morphogenetic alignment of independently specified cell lineages in the leech Helobdella

Détails

ID Serval
serval:BIB_6E534FD0EAE7
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
An axial domain of HOM/Hox gene expression is formed by morphogenetic alignment of independently specified cell lineages in the leech Helobdella
Périodique
Development
Auteur(s)
Nardelli-Haefliger  D., Bruce  A. E., Shankland  M.
ISSN
0950-1991 (Print)
Statut éditorial
Publié
Date de publication
07/1994
Volume
120
Numéro
7
Pages
1839-49
Notes
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S. --- Old month value: Jul
Résumé
The homeobox gene Lox2, a member of the HOM/Hox gene class, is expressed in a restricted domain along the anteroposterior (A-P) body axis of the leech Helobdella. The segmental tissues of the leech embryo arise from the parallel merger of five distinct and bilaterally paired cell lineages generated by embryonic stem cells or teloblasts. Injection of cell lineage tracers coupled with anti-LOX2 immunochemistry reveals that all five teloblast lineages generate central nervous system neurons that express the LOX2 protein, and that each lineage expresses LOX2 within a similar domain of body segments. Some lineally identified neurons display anti-LOX2 immunoreactivity over the entire expression domain, but the OM7 neuron has a distinctively high level of LOX2 expression, which is restricted to the seventh midbody ganglion. To ascertain the role of positional information in the axial patterning of LOX2 expression, we performed focal cell ablations that displaced one or another of the teloblast lineages out of segmental register with the other axial tissues. Such displacements brought about a corresponding shift in the LOX2 expression of the perturbed lineage, and had little or no effect on the LOX2 expression of the other, unperturbed lineages. This result indicates that the axial domain of LOX2 expression is not specified by positional cues acting coordinately across the various teloblast lineages, nor would it seem that the expression domain is imprinted from one lineage to the others. Rather, the different teloblast lineages acquire their axial patterns independently, and secondarily bring these patterns into alignment along the A-P axis through a process of morphogenetic assembly.
Mots-clé
Animals Cell Differentiation/physiology Gene Expression/physiology Genes, Homeobox/*genetics Immunoblotting Immunohistochemistry Leeches/embryology/*genetics Microscopy, Fluorescence Morphogenesis/genetics Neurons/cytology Stem Cells/*physiology
Pubmed
Web of science
Création de la notice
25/01/2008 15:35
Dernière modification de la notice
20/08/2019 15:27
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