Inhibition of heterologous DNA replication by the MVMp nonstructural NS-1 protein: identification of a target sequence.

Details

Serval ID
serval:BIB_6D91C79584DA
Type
Article: article from journal or magazin.
Collection
Publications
Title
Inhibition of heterologous DNA replication by the MVMp nonstructural NS-1 protein: identification of a target sequence.
Journal
Virology
Author(s)
Tenenbaum L., Dupont F., Spegelaere P., Zentilin L., Norio P., Giacca M., Riva S., Falaschi A., Rommelaere J.
ISSN
0042-6822 (Print)
ISSN-L
0042-6822
Publication state
Published
Issued date
1993
Volume
197
Number
2
Pages
630-641
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov'tPublication Status: ppublish
Abstract
The nonstructural protein NS-1 of minute virus of mice (MVMp), an autonomous parvovirus, trans-inhibits the replication of a chimeric plasmid containing the SV40 origin of replication (ori) embedded in the MVMp genome. It appears that a 157-bp 5' proximal sequence of MVMp DNA is sufficient, in the presence of NS-1, to cause the inhibition of DNA replication driven by the SV40 ori placed on the same molecule. This effect is not dependent on the orientation of the MVMp target sequence and results from both a reduced level of utilization of SV40 ori and the blockage of progressing replication forks at the level of the target. Furthermore, replication driven by Epstein-Barr virus origin (oriP) is trans-inhibited by MVMp but this inhibition does not require the presence of parvoviral sequences in cis. On the basis of sequence homologies between EBV oriP and MVMp 5' terminal sequence, it is proposed that the direct or indirect interaction of NS-1 with parvovirus-like sequences present in heterologous viral and possibly also cellular genomes may result in an inhibition of DNA replication.
Keywords
Animals, Base Sequence, Cell Line, DNA Replication/drug effects, DNA, Recombinant/biosynthesis, DNA, Viral/biosynthesis, DNA, Viral/genetics, Herpesvirus 4, Human/genetics, Herpesvirus 4, Human/growth & development, Humans, Minute virus of mice/genetics, Minute virus of mice/growth & development, Molecular Sequence Data, Regulatory Sequences, Nucleic Acid/genetics, Sequence Homology, Nucleic Acid, Simian virus 40/genetics, Simian virus 40/growth & development, Viral Nonstructural Proteins/pharmacology
Pubmed
Web of science
Create date
01/04/2011 10:16
Last modification date
20/08/2019 14:27
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