Targeted INO80 enhances subnuclear chromatin movement and ectopic homologous recombination.

Details

Serval ID
serval:BIB_6C24CEFD52A0
Type
Article: article from journal or magazin.
Collection
Publications
Title
Targeted INO80 enhances subnuclear chromatin movement and ectopic homologous recombination.
Journal
Genes and Development
Author(s)
Neumann F.R., Dion V., Gehlen L.R., Tsai-Pflugfelder M., Schmid R., Taddei A., Gasser S.M.
ISSN
1549-5477 (Electronic)
ISSN-L
0890-9369
Publication state
Published
Issued date
2012
Volume
26
Number
4
Pages
369-383
Language
english
Abstract
Chromatin in the interphase nucleus moves in a constrained random walk. Despite extensive study, the molecular causes of such movement and its impact on DNA-based reactions are unclear. Using high-precision live fluorescence microscopy in budding yeast, we quantified the movement of tagged chromosomal loci to which transcriptional activators or nucleosome remodeling complexes were targeted. We found that local binding of the transcriptional activator VP16, but not of the Gal4 acidic domain, enhances chromatin mobility. The increase in movement did not correlate strictly with RNA polymerase II (PolII) elongation, but could be phenocopied by targeting the INO80 remodeler to the locus. Enhanced chromatin mobility required Ino80's ATPase activity. Consistently, the INO80-dependent remodeling of nucleosomes upon transcriptional activation of the endogenous PHO5 promoter enhanced chromatin movement locally. Finally, increased mobility at a double-strand break was also shown to depend in part on the INO80 complex. This correlated with increased rates of spontaneous gene conversion. We propose that local chromatin remodeling and nucleosome eviction increase large-scale chromatin movements by enhancing the flexibility of the chromatin fiber.
Keywords
Cell Nucleus/metabolism, Chromatin/metabolism, Chromatin Assembly and Disassembly, DNA Breaks, Double-Stranded, Homologous Recombination, Protein Transport, Saccharomyces cerevisiae/genetics, Saccharomyces cerevisiae/metabolism, Saccharomyces cerevisiae Proteins/metabolism
Pubmed
Web of science
Open Access
Yes
Create date
12/02/2014 16:09
Last modification date
20/08/2019 14:26
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