Application of microtitre plates and fluorescence reading to shorten handling of Phadezym RAST and Phadezym IgE PRIST
Details
Serval ID
serval:BIB_69A0EC3EB5FD
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Application of microtitre plates and fluorescence reading to shorten handling of Phadezym RAST and Phadezym IgE PRIST
Journal
Clinical Allergy
ISSN
0009-9090 (Print)
Publication state
Published
Issued date
05/1986
Volume
16
Number
3
Pages
231-9
Notes
Comparative Study
Journal Article --- Old month value: May
Journal Article --- Old month value: May
Abstract
To enable shorter and more convenient testing, the Phadezym RAST and Phadezym IgE PRIST procedures for the determination of specific and total IgE were modified in three ways: (i) allergen-coupled paper discs were tested in microtitre wells; (ii) the incubation times were reduced to 1 hr with serum and 2 hr with the anti-IgE by shaking the plates at room temperature; and (iii) the fluorogenic substrate used reduced the development time to 15 min. Determination of IgE antibody specific for fifteen inhalation allergens by the modified fluorescence test (FEIA) and by the conventional Phadezym RAST (EIA) was performed on the serum of thirty-two patients suffering from asthma/rhinitis: correlation studies for these sera showed that 96.1% of the results fell in the same class. In these patients, both FEIA and EIA detected the same proportion of skin-prick tests (SPT) positive results (67%). With the FEIA, 4/165 (2.4%) class 1 results were found in eleven non-atopic subjects (symptom free, fifteen negative SPT, total IgE lower than 80 kU/l), compared to 1/165 (0.6%) with the EIA. In twenty cord sera, both FEIA and EIA found 4/300 (1.3%) class 1 results. For the determination of total serum IgE, the microtitre FEIA showed a detection limit of 0.5 kU/l and an excellent correlation with Phadezym IgE PRIST (n = 66 serum, r = 0.99). These data indicate that the adaptation of Phadezym RAST and Phadezym IgE PRIST to microtitre plates and fluorescence technology has resulted in a time-saving and easy to perform within-day assay which provided results as reproducible, sensitive and specific as those of the conventional procedure.
Keywords
Adolescent
Adult
Asthma/diagnosis
Child
Evaluation Studies
Female
Fluorescent Dyes
Galactosides
Humans
Hymecromone/analogs & derivatives
Immunoglobulin E/analysis
Male
Middle Aged
Radioallergosorbent Test/instrumentation/*methods
Radioimmunoassay/*methods
Radioimmunosorbent Test/instrumentation/*methods
Rhinitis/diagnosis
Skin Tests
Pubmed
Web of science
Create date
28/01/2008 12:58
Last modification date
20/08/2019 15:24