Role of the cellular factor CTCF in the regulation of bovine leukemia virus latency and three-dimensional chromatin organization.

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State: Public
Version: Final published version
License: CC BY-NC 4.0
Serval ID
serval:BIB_67FC332D016D
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Role of the cellular factor CTCF in the regulation of bovine leukemia virus latency and three-dimensional chromatin organization.
Journal
Nucleic acids research
Author(s)
Bellefroid M., Rodari A., Galais M., Krijger PHL, Tjalsma SJD, Nestola L., Plant E., Vos ESM, Cristinelli S., Van Driessche B., Vanhulle C., Ait-Ammar A., Burny A., Ciuffi A., de Laat W., Van Lint C.
ISSN
1362-4962 (Electronic)
ISSN-L
0305-1048
Publication state
Published
Issued date
08/04/2022
Peer-reviewed
Oui
Volume
50
Number
6
Pages
3190-3202
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Abstract
Bovine leukemia virus (BLV)-induced tumoral development is a multifactorial phenomenon that remains incompletely understood. Here, we highlight the critical role of the cellular CCCTC-binding factor (CTCF) both in the regulation of BLV transcriptional activities and in the deregulation of the three-dimensional (3D) chromatin architecture surrounding the BLV integration site. We demonstrated the in vivo recruitment of CTCF to three conserved CTCF binding motifs along the provirus. Next, we showed that CTCF localized to regions of transitions in the histone modifications profile along the BLV genome and that it is implicated in the repression of the 5'Long Terminal Repeat (LTR) promoter activity, thereby contributing to viral latency, while favoring the 3'LTR promoter activity. Finally, we demonstrated that BLV integration deregulated the host cellular 3D chromatin organization through the formation of viral/host chromatin loops. Altogether, our results highlight CTCF as a new critical effector of BLV transcriptional regulation and BLV-induced physiopathology.
Keywords
CCCTC-Binding Factor/metabolism, Chromatin, Leukemia Virus, Bovine/genetics, Leukemia Virus, Bovine/metabolism, Promoter Regions, Genetic, Terminal Repeat Sequences/genetics, Virus Latency
Pubmed
Web of science
Open Access
Yes
Create date
07/03/2022 10:49
Last modification date
25/01/2024 7:37
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