Phospholipids enhance the binding of peptides to class II major histocompatibility molecules.

Details

Serval ID
serval:BIB_67C22CA4AC54
Type
Article: article from journal or magazin.
Collection
Publications
Title
Phospholipids enhance the binding of peptides to class II major histocompatibility molecules.
Journal
Proceedings of the National Academy of Sciences of the United States of America
Author(s)
Roof R.W., Luescher I.F., Unanue E.R.
ISSN
0027-8424
Publication state
Published
Issued date
1990
Peer-reviewed
Oui
Volume
87
Number
5
Pages
1735-1739
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, P.H.S.
Publication Status: ppublish
Abstract
The binding of a lysozyme and ovalbumin peptide to purified class II major histocompatibility molecules in detergents was increased by the addition of certain lipids. Natural lipids from B lymphoma cells enhanced the binding and so did phosphatidylcholine, phosphatidylserine, phosphatidylinositol, and cardiolipin. Phosphatidylethanolamine, sphingomyelin, and cholesterol had no effect. There was no major difference between the effects of a phospholipid and its lyso derivative. As studied with phosphatidylcholine, the increase in peptide binding was also dependent on the fatty acid composition of the lipid. The binding affinity was increased 10- to 50-fold in the presence of lipid as a result of an increase in the association rate while the off-rate remained essentially unchanged. Our results suggest that lipids, directly or indirectly, induce conformational changes in class II molecules that favor their peptide-binding property.
Keywords
Amino Acid Sequence, B-Lymphocytes/immunology, Cell Line, Histocompatibility Antigens Class II/isolation &amp, purification, Humans, Kinetics, Lymphoma/immunology, Molecular Sequence Data, Ovalbumin/immunology, Ovalbumin/metabolism, Peptides/immunology, Peptides/metabolism, Phospholipids/pharmacology, Protein Binding, Structure-Activity Relationship
Pubmed
Web of science
Create date
28/01/2008 11:19
Last modification date
20/08/2019 14:23
Usage data