Ultrastructural distribution of the death-domain-containing MyD88 protein in HeLa cells
Details
Serval ID
serval:BIB_67063A4F9A3C
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Ultrastructural distribution of the death-domain-containing MyD88 protein in HeLa cells
Journal
Experimental Cell Research
ISSN
0014-4827 (Print)
Publication state
Published
Issued date
08/1998
Volume
243
Number
1
Pages
67-75
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Aug 25
Research Support, Non-U.S. Gov't --- Old month value: Aug 25
Abstract
MyD88, a protein implicated in interleukin-1 signaling, was localized in HeLa cells transiently transfected with an epitope-tagged (flag) version of MyD88. Overexpression of MyD88 can induce apoptosis. We have analyzed the fine structural intracellular distribution of MyD88 using immunoelectron microscopy. MyD88 is localized to the nucleus and to the cytoplasm as revealed by immunofluorescence visualization. Ultrastructural immunocytochemistry shows that, in the cytoplasm, this protein is associated with fibrillar aggregates containing beta-actin. In the nucleus, MyD88 was found in fibrillar domains present only in cells not yet displaying morphological signs of apoptosis. These domains are not derived from nucleoli and do not constitute an accumulation site of splicing factors. We suggest that such structures could be involved in the formation of the apoptotic bodies and/or in the modification of the nuclear structure and of nucleocytoplasmic trafficking during apoptosis.
Keywords
Adaptor Proteins, Signal Transducing
*Antigens, Differentiation
Apoptosis/*genetics
Cell Nucleus/metabolism
Cytoplasm/metabolism
Cytoskeletal Proteins/metabolism
Fluorescent Antibody Technique, Indirect
Gene Expression
Hela Cells/*metabolism/ultrastructure
Humans
Immunohistochemistry
Microscopy, Immunoelectron
Myeloid Differentiation Factor 88
Proteins/genetics/*metabolism
*Receptors, Immunologic
Transfection
Pubmed
Web of science
Create date
24/01/2008 16:19
Last modification date
20/08/2019 15:22