A limited role for beta-selection during gamma delta T cell development.

Détails

ID Serval
serval:BIB_6599
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
A limited role for beta-selection during gamma delta T cell development.
Périodique
Journal of Immunology
Auteur(s)
Wilson A., MacDonald H.R.
ISSN
0022-1767
Statut éditorial
Publié
Date de publication
1998
Peer-reviewed
Oui
Volume
161
Numéro
11
Pages
5851-5854
Langue
anglais
Résumé
T cells belong to two distinct lineages expressing either alpha beta or gamma delta TCR. During alpha beta T cell development, it is clearly established that productive rearrangement at the TCR beta locus in immature precursor cells leads to the expression of a pre-TCR complex. Signaling through the pre-TCR results in the selective proliferation and maturation of TCR beta+ cells, a process that is known as beta-selection. However, the potential role of beta-selection during gamma delta T cell development is controversial. Whereas PCR-RFLP and sequencing techniques have provided evidence for a bias toward in-frame VDJ beta rearrangements in gamma delta cells (consistent with beta-selection), gamma delta cells apparently develop normally in mice that are unable to assemble a pre-TCR complex due to a deficiency in TCR beta or pT alpha genes. In this report, we have directly addressed the physiologic significance of beta-selection during gamma delta cell development in normal mice by quantitating intracellular TCR beta protein in gamma delta cells and correlating its presence with cell cycle status. Our results indicate that beta-selection plays a significant (although limited) role in gamma delta cell development by selectively amplifying a minor subset of gamma delta precursor cells with productively rearranged TCR beta genes.
Mots-clé
Animals, Cell Cycle/genetics, Cell Cycle/immunology, Cell Differentiation/genetics, Cell Differentiation/immunology, Cell Division/genetics, Cell Division/immunology, Cell Lineage/genetics, Cell Lineage/immunology, Female, Gene Rearrangement, beta-Chain T-Cell Antigen Receptor, Intracellular Fluid/metabolism, Lymphocyte Count, Mice, Mice, Inbred C57BL, Receptors, Antigen, T-Cell, alpha-beta/biosynthesis, Receptors, Antigen, T-Cell, alpha-beta/genetics, Receptors, Antigen, T-Cell, gamma-delta/biosynthesis, Receptors, Antigen, T-Cell, gamma-delta/physiology, T-Lymphocyte Subsets/cytology, T-Lymphocyte Subsets/immunology, Thymus Gland/cytology, Thymus Gland/metabolism
Pubmed
Web of science
Création de la notice
19/11/2007 12:43
Dernière modification de la notice
20/08/2019 14:21
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