Analysis of streptomycin-resistance of Escherichia coli mutants

Details

Serval ID
serval:BIB_656A3B3E1CD4
Type
Article: article from journal or magazin.
Collection
Publications
Title
Analysis of streptomycin-resistance of Escherichia coli mutants
Journal
Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression
Author(s)
Bonny Christophe, Montandon Paul-Etienne, Marc-Martin Sophie, Stutz Erhard
ISSN
0167-4781
ISSN-L
1879-2634
Publication state
Published
Issued date
06/1991
Peer-reviewed
Oui
Volume
1089
Number
2
Pages
213-219
Language
english
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Jun 13
Abstract
We previously reported about Escherichia coli transformation experiments yielding streptomycin-resistant cells carrying a C912 to T transition in a plasmid-born 16S rRNA gene. These experiments were based on results obtained with streptomycin-resistant Euglena chloroplasts bearing an equivalent mutation in the single chloroplast 16S rRNA gene. We extended this study and transformed E. coli with plasmid constructs having a mutated 16S rRNA gene at position 914 (A to C) or a double mutation at positions 912 and 888 (C to T:G to A) or a mutation in the S12 gene (Lys-42 to Thr). We tested the transformed cells before and after a screening procedure in the presence of streptomycin. We find that the plasmid-born mutations protect colonies against a short streptomycin exposure, but ribosomes carrying mutated 16S rRNA do not significantly reduce codon misreading in vitro. However, ribosomes isolated from transformed cells after the screening procedure resist misreading. These ribosomes have acquired a second mutation in the S12 protein as shown in one case by sequencing and by transformation experiments. Furthermore, we show that the A914 to C mutation prevents (strongly reduces) base methylation in the central domain of 16S rRNA.
Keywords
Base Sequence Chloroplasts Drug Resistance, Microbial/*genetics Escherichia coli/drug effects/*genetics Molecular Sequence Data Mutagenesis, Site-Directed *Mutation Plasmids RNA, Bacterial/genetics RNA, Ribosomal, 16S/genetics Ribosomal Proteins/genetics Streptomycin/*pharmacology, Biophysics, Genetics, Biochemistry, Structural Biology
Pubmed
Web of science
Create date
25/01/2008 14:16
Last modification date
20/08/2019 14:21
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