Molecular characterization of the human interferon-gamma receptor: analysis of polymorphism and glycosylation.

Details

Serval ID
serval:BIB_636FBD6DA1B4
Type
Article: article from journal or magazin.
Collection
Publications
Title
Molecular characterization of the human interferon-gamma receptor: analysis of polymorphism and glycosylation.
Journal
Journal of Interferon Research
Author(s)
Mao C., Aguet M., Merlin G.
ISSN
0197-8357 (Print)
ISSN-L
0197-8357
Publication state
Published
Issued date
1989
Volume
9
Number
6
Pages
659-669
Language
english
Abstract
Different molecular masses have been assigned to the human interferon-gamma receptor (HuIFN-gamma-R) by several authors. After extensive purification from Raji cells, this receptor was shown in a previous work to consist of two major protein species with molecular masses of 92 kD and 50 kD, as revealed by SDS-PAGE. We show here that the 50-kD band is most probably a degradation product of the 92-kD band due to a trypsin-like protease active during the purification process. The native protein of Raji cells seems, therefore, to have a molecular mass of 92 kD. The same molecular mass was found with Colo 205 cells (derived from a colon carcinoma). However, in conditions where degradation does not occur, the HuIFN-gamma-R shows a certain polymorphism: in IM-9 cells, another B-cell line, two bands exist with molecular masses of 95 kD and 85 kD, and in Wish cells, an amnion-derived cell line, one (or two) band(s) can be detected around 87 kD. This polymorphism is due at least in part to a variable extent of N-glycosylation from line to line and also within the same line, since after tunicamycin treatment of the Raji, IM-9, and Wish cells, very similar bands are obtained with a molecular mass of 72 kD.
Keywords
Blotting, Western, Cell Line, Glycosylation, Humans, Interferon-gamma/metabolism, Molecular Weight, Polymorphism, Genetic, Receptors, Immunologic/drug effects, Receptors, Immunologic/genetics, Receptors, Interferon, Tunicamycin/pharmacology
Pubmed
Web of science
Create date
28/01/2008 11:36
Last modification date
20/08/2019 14:20
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