Activation by IL-1 of bovine articular chondrocytes in culture within a 3D collagen-based scaffold. An in vitro model to address the effect of compounds with therapeutic potential in osteoarthritis.

Détails

ID Serval
serval:BIB_5E23644A0A4F
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Activation by IL-1 of bovine articular chondrocytes in culture within a 3D collagen-based scaffold. An in vitro model to address the effect of compounds with therapeutic potential in osteoarthritis.
Périodique
Osteoarthritis and Cartilage / Oars, Osteoarthritis Research Society
Auteur(s)
Cortial D., Gouttenoire J., Rousseau C.F., Ronzière M.C., Piccardi N., Msika P., Herbage D., Mallein-Gerin F., Freyria A.M.
ISSN
1063-4584 (Print)
ISSN-L
1063-4584
Statut éditorial
Publié
Date de publication
2006
Volume
14
Numéro
7
Pages
631-640
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
OBJECTIVE: To determine the best protocol for the preparation of a tissue-engineered cartilage to investigate the potential anti-arthritic and/or anti-osteoarthritic effects of drugs.
METHODS: Calf articular chondrocytes, seeded in collagen sponges were grown in culture for up to 1 month. At day 14 cultures received interleukin (IL)-1beta (ranging from 0.1 to 20 ng/ml) for 1 to 3 days. Analyses of gene expression for extracellular matrix proteins, collagen-binding integrins, matrix metalloproteinases (MMPs), aggrecanases, TIMPs, IL-1Ra and Ikappa-Balpha were carried out using real-time polymerase chain reaction (PCR). Metalloproteinase activities were analysed in the culture medium using both zymography and fluorogenic peptide substrates.
RESULTS: We selected a culture for 15 or 17 days with collagen sponges seeded with 10(7) chondrocytes showing a minimal cell proliferation, a maximal sulphated glycosaminoglycan (sGAG) deposition and a high expression of COL2A1, aggrecan and the alpha10 integrin sub-unit and low expression of COL1A2 and the alpha11 integrin sub-unit. In the presence of 1 ng/ml IL-1beta, we observed at day 15 up-regulations of 450-fold for MMP-1, 60-fold for MMP-13, 54-fold for ADAMTS-4 and MMP-3 and 10-fold for ADAMTS-5 and IL-1Ra. Down-regulations of 2.5-fold for COL2A1 and aggrecan were observed only at day 17. At the protein level a dose-dependent increase of total MMP-1 and MMP-13 was noted with less than 15% in the active form.
CONCLUSIONS: This in vitro model of chondrocyte culture in three dimensional (3D) seems well adapted to investigate the responses of these cells to inflammatory cytokines and to evaluate the potential anti-inflammatory effects of drugs.
Mots-clé
ADAM Proteins/biosynthesis, Aggrecans/metabolism, Animals, Cartilage, Articular/drug effects, Cattle, Chondrocytes/drug effects, Collagen/metabolism, Integrins/metabolism, Interleukin-1/pharmacology, Matrix Metalloproteinases/biosynthesis, Osteoarthritis/drug therapy, Tissue Engineering/methods, Tissue Inhibitor of Metalloproteinases/metabolism
Pubmed
Open Access
Oui
Création de la notice
01/10/2015 16:29
Dernière modification de la notice
20/08/2019 15:16
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