In vivo <sup>13</sup>C MRS in the mouse brain at 14.1 Tesla and metabolic flux quantification under infusion of [1,6-<sup>13</sup>C<sub>2</sub>]glucose.

Détails

ID Serval
serval:BIB_5C44786165AA
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
In vivo <sup>13</sup>C MRS in the mouse brain at 14.1 Tesla and metabolic flux quantification under infusion of [1,6-<sup>13</sup>C<sub>2</sub>]glucose.
Périodique
Journal of cerebral blood flow and metabolism
Auteur(s)
Lai M., Lanz B., Poitry-Yamate C., Romero J.F., Berset C.M., Cudalbu C., Gruetter R.
ISSN
1559-7016 (Electronic)
ISSN-L
0271-678X
Statut éditorial
Publié
Date de publication
10/2018
Peer-reviewed
Oui
Volume
38
Numéro
10
Pages
1701-1714
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
In vivo <sup>13</sup> C magnetic resonance spectroscopy (MRS) enables the investigation of cerebral metabolic compartmentation while, e.g. infusing <sup>13</sup> C-labeled glucose. Metabolic flux analysis of <sup>13</sup> C turnover previously yielded quantitative information of glutamate and glutamine metabolism in humans and rats, while the application to in vivo mouse brain remains exceedingly challenging. In the present study, <sup>13</sup> C direct detection at 14.1 T provided highly resolved in vivo spectra of the mouse brain while infusing [1,6- <sup>13</sup> C <sub>2</sub> ]glucose for up to 5 h. <sup>13</sup> C incorporation to glutamate and glutamine C4, C3, and C2 and aspartate C3 were detected dynamically and fitted to a two-compartment model: flux estimation of neuron-glial metabolism included tricarboxylic acid cycle (TCA) flux in astrocytes (V <sub>g</sub>  = 0.16 ± 0.03 µmol/g/min) and neurons (V <sub>TCA</sub> <sup>n </sup> = 0.56 ± 0.03 µmol/g/min), pyruvate carboxylase activity (V <sub>PC</sub>  = 0.041 ± 0.003 µmol/g/min) and neurotransmission rate (V <sub>NT</sub>  = 0.084 ± 0.008 µmol/g/min), resulting in a cerebral metabolic rate of glucose (CMR <sub>glc</sub> ) of 0.38 ± 0.02 µmol/g/min, in excellent agreement with that determined with concomitant <sup>18</sup> F-fluorodeoxyglucose positron emission tomography ( <sup>18</sup> FDG PET).We conclude that modeling of neuron-glial metabolism in vivo is accessible in the mouse brain from <sup>13</sup> C direct detection with an unprecedented spatial resolution under [1,6- <sup>13</sup> C <sub>2</sub> ]glucose infusion.
Mots-clé
Animals, Brain/metabolism, Carbon Isotopes/analysis, Carbon Isotopes/metabolism, Glucose/analysis, Glucose/metabolism, Glutamic Acid/analysis, Glutamic Acid/metabolism, Glutamine/analysis, Glutamine/metabolism, Magnetic Resonance Spectroscopy/methods, Male, Mice, Models, Neurological, Neuroglia/metabolism, Neurons/metabolism, 13C MRS, glucose metabolism, metabolic modelling, mouse brain
Pubmed
Web of science
Open Access
Oui
Création de la notice
02/11/2017 15:08
Dernière modification de la notice
20/08/2019 15:14
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