Cohesin and the nucleolus constrain the mobility of spontaneous repair foci.

Details

Serval ID
serval:BIB_578853CAAAD4
Type
Article: article from journal or magazin.
Collection
Publications
Title
Cohesin and the nucleolus constrain the mobility of spontaneous repair foci.
Journal
EMBO Reports
Author(s)
Dion V., Kalck V., Seeber A., Schleker T., Gasser S.M.
ISSN
1469-3178 (Electronic)
ISSN-L
1469-221X
Publication state
Published
Issued date
2013
Volume
14
Number
11
Pages
984-991
Language
english
Abstract
The regulation of chromatin mobility in response to DNA damage is important for homologous recombination in yeast. Anchorage reduces rates of recombination, whereas increased chromatin mobility correlates with more efficient homology search. Here we tracked the mobility and localization of spontaneous S-phase lesions bound by Rad52, and find that these foci have reduced movement, unlike enzymatically induced double-strand breaks. Moreover, spontaneous repair foci are positioned in the nuclear core, abutting the nucleolus. We show that cohesin and nucleolar integrity constrain the mobility of these foci, consistent with the notion that spontaneous, S-phase damage is preferentially repaired from the sister chromatid.
Keywords
DNA damage, Rad52 foci, chromatin dynamics, cohesin, nucleolus
Pubmed
Web of science
Open Access
Yes
Create date
12/02/2014 15:29
Last modification date
20/08/2019 14:11
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