Molecular defects in the non-expressed H-2 E alpha genes of the f and q haplotypes.

Détails

ID Serval
serval:BIB_56EBB26575D4
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Molecular defects in the non-expressed H-2 E alpha genes of the f and q haplotypes.
Périodique
Journal of Immunology
Auteur(s)
Vu T.H., Begovich A.B., Tacchini-Cottier F.M., Jones P.P.
ISSN
0022-1767 (Print)
ISSN-L
0022-1767
Statut éditorial
Publié
Date de publication
1989
Volume
142
Numéro
8
Pages
2936-2942
Langue
anglais
Résumé
The expression and sequences of the mouse MHC class II genes, E alpha f and E alpha q, have been studied to determine the molecular basis for their defective expression. Previous work in our laboratory showed that H-2f and H-2q mice produce no detectable E alpha protein. Northern blot analysis confirms previous results showing normal amounts of E alpha f message and a 100-fold reduction in E alpha q mRNA. Despite that observation, the rates of transcription of both defective E alpha genes, measured by nuclear run-on transcription assays, are comparable to that of the normally expressed E alpha k gene. The nucleotide sequences of the E alpha f and E alpha q genes reveal mutations generating premature translation stop codons in both genes. A single base substitution has created the stop codon TGA at amino acid -2 in the E alpha f leader sequence. A nucleotide insertion at codon 64 in the second exon of the E alpha q gene results in a frame shift and a premature stop codon at amino acid residue 69 of a mature E alpha protein. The low steady state level of E alpha q mRNA may be correlated with the unusual size distribution of the RNA, possibly due to altered RNA processing.
Mots-clé
Animals, Base Sequence, H-2 Antigens/genetics, Haplotypes, Mice, Molecular Sequence Data, Mutation, RNA, Messenger/analysis, Receptors, Antigen, T-Cell/genetics, Transcription, Genetic
Pubmed
Web of science
Création de la notice
24/01/2008 16:08
Dernière modification de la notice
20/08/2019 15:11
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